Course catalogue doctoral education - VT24
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| Title | Immunometabolism: A Hands-on Course on a Frontrunning Research Field |
|---|---|
| Course number | 5744 |
| Programme | 1-Ingår i flera program |
| Language | English |
| Credits | 1.5 |
| Date | 2024-03-25 -- 2024-03-29 | Responsible KI department | Institutionen för medicin, Solna |
| Specific entry requirements | Basic knowledge of immunology or cellular metabolism |
| Purpose of the course | This course aims to convey knowledge on the regulation of metabolism in immune cells. Moreover, the course emphasizes the cutting-edge research on cellular and mitochondria energy production, and includes hands-on experiments and data evaluation using a Seahorse flux analyzer. |
| Intended learning outcomes | After successful completion of this course students are expected to be able to:
- Identify the basic concepts of energy production and metabolism in different immune cells. - Understand the molecular mechanisms underlying energy metabolism changes in response to various immunological stimuli. - Argue and judge scientific data about immunometabolism and methods applied to respond to specific questions. - Analyze the two major metabolic pathways of the cell: glycolysis and oxidative phosphorylation using the Seahorse flux analyzer. |
| Contents of the course | The behavior of different immune cells is largely determined by the status of their intracellular energy metabolism and external signals which activate a metabolic reprogramming.
The course will have an introduction on the different immune cells and their role in disease. The course will then specifically focus on the branch of metabolism, such as oxidative phosphorylation and glycolysis, which underlie immune cell functioning in health and disease states (e.g. infections, cancer and cardiometabolic diseases). The course includes a hands-on module where the students will learn how to measure and critically analyze cellular metabolism using Seahorse flux analyzer in different immune cells. |
| Teaching and learning activities | Lectures, laboratory practice and group discussion. |
| Compulsory elements | All course activities are mandatory. Absence can be compensated for in exceptional circumstances by a written assignment in agreement with the course leader. |
| Examination | A course participant has to achieve all intended learning outcomes for the course in order to pass the course. This is tested at the end of the course by:
- Individual presentations by the students on specific metabolic data in immune cells chosen by the participants. - Analysis of the metabolic data from the seahorse flux analyzer (Seahorse) generated during the laboratory practice. |
| Literature and other teaching material | Subject-specific literature (relevant scientific papers) will be distributed one-week before the course. For basic immunological knowledge refer to one of the later editions of the following textbooks for information regarding different cell types: ‘Cellular and Molecular Immunology’ - Abul K. Abbas, 'Immunobiology' - Janeway; 'Immunology' – Kuby. For information about bionergetic and cell metabolism please refer to “Bionergetics 4” Nicholls and Ferguson. |
| Number of students | 8 - 30 |
| Selection of students | Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) start date of doctoral studies (priority given to earlier start date) |
| More information | The course will be hold from monday to friday (day of the examination)
The lectures will take place Mainly at the Rolf Luft Centrum L103 Karolinska Hospital
The laboratory practices will take place in the same structure.
This course is given jointly by the doctoral programmes Cardiovascular Research (CVR), Allergy, immunology and inflammation (Aii) and Metabolism and Endocrinology (MetEndo). See: https://staff.ki.se/doctoral-programmes |
| Additional course leader | Itziar Martinez Gonzalez |
| Latest course evaluation | Course evaluation report |
| Course responsible |
Noah Moruzzi Institutionen för molekylär medicin och kirurgi noah.moruzzi@ki.se |
| Contact person |
Noah Moruzzi Institutionen för molekylär medicin och kirurgi noah.moruzzi@ki.se Itziar Martinez Gonzalez Institutionen för mikrobiologi, tumör- och cellbiologi itziar.martinez.gonzalez@ki.se |
