Kurskatalog forskarutbildning - VT22

  • Ansökan kan ske mellan 2021-10-15 och 2021-11-15
Application closed
Skriv ut
Titel Mass spectrometry-based proteomics: When and How.
Kursnummer 2522
Program 1-Ingår i flera program
Språk Engelska
Antal högskolepoäng 3.0
Datum 2021-11-08 -- 2021-11-19
Kursansvarig institution Institutionen för onkologi-patologi
Särskild behörighet
Kursens syfte The aim of this course is to give an overview of mass spectrometry based proteomics for researchers who would like to be able to apply these techniques in their own research.
Kursens lärandemål After completed the course, the student will be able to

-Define and apply common proteomics terminology
-Recognize the principles of the most common proteomics techniques
-Understand the mass spectrometry based proteomics workflow: Experimental design, sample preparation and selection of techniques
-Understand proteomics to such an extent that he/she will be able to utilize proteomics core facilities or collaborate with proteomics researchers.
-Evaluate the quality of protein and peptide identifications.
-Demonstrate how modern proteomics is applied to clinical research and to evaluate when proteomics research could be beneficial to incorporate in research projects.
-Design their own research project by mass spectrometry based proteomics.
Kursens innehåll Lectures and workshops on
-Overview of proteomics
-The proteomics workflow
-Basic of separation sciences: Protein and peptides
-Introduction to Mass spectrometry
-Experimental design
-Sample preparation, immunoprecipitation and enrichment strategy
-Quantitative and qualitative proteomics by mass spectrometry
-Global and targeted proteomics
-Human protein atlas and Biological validation
-Clinical applications

A practical laboratory exercise using mass spectrometry based proteomics

This course is focusing on proteomics technologies and applications, for proteomics data analysis we recommend our KI doctoral course ""Omics data analysis: From quantitative data to biological information""
Arbetsformer The course contains lectures, seminars, and a practical laboratory exercise using proteomics techniques. The students will plan their own project as a case study as well make an oral laboratory report.
Obligatoriska moment -Attendance at lectures and the practical laboratory exercise.
-Attendance at examination seminar and hand in the written examination assignment.
-Extra written assignments can be used to compensate absence.

The students are recommended to read literatures listed below in advance of the course.
Examination -Students shall describe a proteomics project (beneficially related to their research) including different aspects from the proteomics workflow. This is handed in as a written exam.
-The students shall perform an oral laboratory report
Kurslitteratur och övriga läromedel The students are recommended to read in advance of the course. (Will be listed on the learning platform)

* Aebersold R, Mann M. Nature. Mass-spectrometric exploration of proteome structure and function.
Nature 2016 537(7620):347-55.

* Matthias Mann, Nils A. Kulak, Nagarjuna Nagaraj, and Jurgen Cox. The Coming Age of Complete, Accurate,
and Ubiquitous Proteomes. Molecular Cell 2013 49; 583-590.

* F. Maarten Altelaar, Javier Munoz and Albert J. R. Heck. Next-generation proteomics: towards an integrative
view of proteome dynamics. Nat Rev Genetics. 2013. 14; 35-48

* Cox J. and Mann M. Quantitative, High-Resolution Proteomics for Data-Driven Systems Biology. Annu. Rev.
Biochem. 2011. 80; 273-99

* Domon B. and Aebersold R. Options and considerations when selecting a quantitative proteomics strategy.
Nat Biotechnol. 2010 28(7); 710-721.

* Gstaiger M. and Aebersold R. Applying mass spectrometry-based proteomics to genetics, genomics and
network biology. Nat Rev Genetics. 2009. 10; 617-627

Supplementary literature will help to understand lectures and design of your project: Will be listed on the learning platform and supplied during the course.

Antal studenter 12 - 24
Urval av studenter Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) start date of doctoral studies (priority given to earlier start date).
Övrig information This course is included in the doctoral programmes Allergy, immunology and inflammation (Aii) and Biology of Infections and Global Health Programme (BIGH). See https://ki.se/en/staff/doctoral-programmes.
Ytterligare kursledare
Senaste kursvärdering Kursvärderingsrapport
Kursansvarig Henrik Johansson
Institutionen för onkologi-patologi

Kontaktpersoner Ghazaleh Assadi
Institutionen för onkologi-patologi