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| Titel | Basic Electron Microscopy for Cell Biologists |
|---|---|
| Kursnummer | 3219 |
| Program | Cellbiologi och genetik |
| Språk | Engelska |
| Antal högskolepoäng | 1.5 |
| Datum | 2021-05-03 -- 2021-05-07 | Kursansvarig institution | Institutionen för cell- och molekylärbiologi |
| Särskild behörighet | |
| Kursens syfte | The students take this course in order to learn about the ways electron microscopy methods are used in the study of biological problems, and to gain knowledge in the theoretical basis of electron microscopy and some practical starting skills in electron microscopy methods. |
| Kursens lärandemål | After the completed course, the students understand and can explain the theoretical basis of electron microscopy techniques, and explain how different kinds of biological research problems are studied using electron microscopy techniques. The students have basic knowledge on the application of different preparation methods, and can critically analyse and relate them to cell biological research questions. The students have basic (novice level) practical knowledge on the different practical preparation techniques. |
| Kursens innehåll | The course introduces students to electron microscopy and the kind of biological problems such as different biological functions and structures that are studied using electron microscopy. The course includes training in basic methods used to do research on biological material such as subcellular structures. The course includes theoretical lectures, discussions and practical sessions on specimen preparation for conventional and cryo-transmission electron microscopy. Most important tissue processing methods for electron microscopy: fixation, dehydration, resin embedding, sectioning, negative staining of ultrathin sections. There are demonstrations on preprative methods in electron microscopy. Some Cryo-EM techniques will be demonstrated. |
| Arbetsformer | The learning and teaching activities include talks (lectures), discussions, laboratory activities and demonstrations. |
| Obligatoriska moment | The practical laboratories and demonstrations are obligatory. Absence from obligatory moments is regulated by instructions of the course leader. |
| Examination | The outcomes are examined through an individual quiz with multiple choice questions, and a short individual written report/reflection on how the participants can employ EM techniques in their own research. |
| Kurslitteratur och övriga läromedel | Recent articles and different web resources. |
| Antal studenter | 8 - 12 |
| Urval av studenter | Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) start date of doctoral studies (priority given to earlier start date) |
| Övrig information | The course will be held on the Karolinska Institutet's Solna campus. Lectures and final exam will be held in Biomedicum while practicals and demonstrations will be held in the KI 3D-EM facility (Nobels v. 12E). Designated time is 9:00 to 17:00 Monday to Friday including time for individual studies and group work. Assembly on the first day is in Biomedicum B0317 at 0900. The days typically consist of lectures before lunch and practicals/demonstrations in the afternoons. Practicals and demonstrations will include vitrification, cryo-confocal imaging for object identification, cryo-FIB-SEM preparation methods for transmission electron microscopy, cryo-volume imaging using FIB-SEM and cryo-transmission electron microscopy. |
| Ytterligare kursledare | Sergej Masich |
| Senaste kursvärdering | Not available |
| Kursansvarig |
Martin Hällberg Institutionen för cell- och molekylärbiologi Martin.Hallberg@ki.se |
| Kontaktpersoner |
Linda Lindell Institutionen för cell- och molekylärbiologi 08-524 872 90 linda.lindell@ki.se Solnavägen 9 171 77 Stockholm |
