Kurskatalog forskarutbildning - HT22

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Titel Mouse Necropsy
Kursnummer 3036
Program 0-Inte del av forskarutbildningsprogram
Språk Engelska
Antal högskolepoäng 1.0
Noteringar Kursen är en försöksdjurskurs.

Datum 2022-05-23 -- 2022-05-25
Kursansvarig institution Komparativ medicin
Särskild behörighet Students need to have completed a laboratory animal science course on how to carry out scientific procedures on mice i.e. EU Function A or equivalent course.
Kursens syfte This course will provide the students with the theoretical background of performing mouse necropsies, including procedures and methods to preserve organs and tissues for further morphological analysis. The course will primarily be practical. This should ultimately enable the students to perform complete mouse necropsies in a standardized and reproducible way.
Kursens lärandemål After completion of this course, the participants of the course should 1) know how to carry out a necropsy in a mouse according to good veterinary and scientific practice, 2) understand the requirements needed to perform organ sampling through a standardized necropsy protocol. The practical learning outcomes based on a supervised training of mouse necropsies will provide the students with a basic knowledge to describe organ changes, how to document these and preserve the organs in such a way that further analysis can be performed on tissues that show a minimum of autolysis, thus providing optimal conditions for extracting useful information from mice.
Kursens innehåll This course will provide a basic foundation to individuals who, perform research using experimental mouse models, and have to conduct a post-mortem examination to analyze morphological changes either due to experimental manipulation or due to introduced genetic changes. The course will provide a theoretical background to standardized mouse necropsies, sampling of organs, alternative ways of preserving these for further morphological analyses, as well as trimming of the organs and preparing these for sectioning for later evaluation using microscopy. A basic description of terms used to describe gross changes will be provided both in lectures and through recommended literature. The main part of the course will be devoted to practical necropsy training, where the participants will be actively supervised during the training.
Arbetsformer The course will be based on pre-reading material on basic mouse anatomy and necropsy techniques, lectures/webinars on mouse necropsy, sample collection, tissue handling and fixation, practical demonstrations and individual practical training on such issues. Since the ultimate aim is to provide course participants with enough practical training to enable them to perform independent necropsies and tissue sampling this two-day course will be split into 1+1 days with an intermittent period for personal training and reflection. In this way, participants will be given an intensive instruction and training during the first day, with a follow up providing feedback on an individual basis and more supervised training during the second day.
Obligatoriska moment All mouse necropsy sessions and active student participation are compulsory. Missed parts of the course as a consequence of a well-justified absence will need to be compensated after agreement with the course director e.g. with written assignment or in future course editions.
Examination Practical formative assessments will be carried out by direct observation of skills and documentation of actions taken by learners during their training laboratory session. A short answer/multiple choice question final examination will also be held at the end of the course. A pass/fail criteria will be used as a global rate for this course.
Kurslitteratur och övriga läromedel Lecture notes and handouts covering the course contents will be provided.

Recommended reading:
1. V. Popesko, V. Rajtová and J. Horák: A Colour Atlas of the Anatomy of Small Laboratory Animals. Vol. II. Rat, Mouse, Hamster. Wolfe Publishing Ltd., London 1992.
2. Treuting PM and Dintzis SM (Editors) Comparative Anatomy and Histology, Second Edition: A Mouse, Rat, and Human Atlas 2nd Edition
3. Scudamore CL. A Practical Guide to the Histology of the Mouse. Wiley_Blackwell 2014.
4. Iwaki T, Yamashita H, Hayakawa T. A Color Atlas of Sectional Anatomy of the Mouse 1st Edition. Braintree Scientific, 2001.
5. Hedrich H. The Laboratory Mouse. 2nd Edition, Academic Press 2012. (also available as an electronic book via KIB)
6. Capas-Peneda S, Munhoz Morello G, Lamas S, Olsson IAS, Gilbert C. Necropsy protocol for newborn mice. Laboratory Animals. January 2021.

Internet resources and electronic publications:
1. The Virtual Mouse Necropsy. http://www.oocities.org/virtualbiology/
2. Vincenzo Covelli - Guide to the Necropsy of the Mouse. http://eulep.pdn.cam.ac.uk/Necropsy_of_the_Mouse/printable.php
3. Scudamore CL, Busk N, Vowell K. A simplified necropsy technique for mice: making the most of unscheduled deaths. Lab Anim. 2014 Oct;48(4):342-4
4. Slaoui M, Bauchet AL, Fiette L.Tissue Sampling and Processing for Histopathology Evaluation. Methods Mol Biol. 2017;1641:101-114
5. Fiette L1, Slaoui M2, Bauchet AL3. Procedures of Necropsy and Tissue Sampling Methods Mol Biol. 2017;1641:71-100
Antal studenter 6 - 8
Urval av studenter Selection will be based on the relevance of the course syllabus for the applicant's doctoral project (i.e. use of mouse models), which will be according to written motivation. If necessary, additional selection criterium will be used based on the date for registration as a doctoral student (priority given to earlier registration date).
Övrig information Both face-to-face teaching (or live webinars) and hands-on exercises will be held on May 23 & May 25, 2022 between approx. 9am and 5pm. Location: Learning Lab, Nobels väg 16, 3rd floor, Comparative Medicine (Solna).
Ytterligare kursledare Raoul Kuiper, PhD DVM Dipl. ECVP pathology Department of Laboratory Medicine FENO, F52 Karolinska University Hospital Huddinge SE-141 86 Stockholm Telephone: +46858583902 Mail: Raoul.Kuiper@ki.se
Senaste kursvärdering Kursvärderingsrapport
Kursansvarig Rafael Frias
Komparativ medicin
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