Course catalogue doctoral education - VT22

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Title Translational Strategies in Cardiovascular Research
Course number 5528
Programme Kardiovaskulär forskning
Language English
Credits 1.5
Date 2022-04-04 -- 2022-04-08
Responsible KI department Institutionen för molekylär medicin och kirurgi
Specific entry requirements
Purpose of the course The purpose of the course is to enable doctoral students and postdoctoral fellows to obtain in-depth knowledge on new scientific advancements in cardiovascular research, and hands-on experience on methodology/techniques of in vitro, ex vivo, in vivo approaches, as well as biobank data and sequencing data analysis.
Intended learning outcomes At the end of the course the participant should be able to:
- Be able to describe the novel clinical and basic strategies in cardiovascular research, including specific biobank definition and set up approaches, novel in vitro, ex vivo, and in vivo experimental methods.
- Be able to design a preliminary study to observe the pathological alterations in cardiovascular diseases, either as a biomarker or therapeutic targets.
- Have obtained knowledge on how to practically perform, analyze and interpret in vitro, ex vivo experiments for cardiovascular studies (eg. ISH, transfection, quantitative live-cell assaysl).
-Be able to show a basic understanding of how to analyze the data from Chip-seq, single-cell RNA-seq and RNA-seq data (via analytical assignments on example data).
Contents of the course The course is designed to enable the acquisition of theoretical knowledge about translational strategies in cardiovascular research, as well as about the applications of novel tools and models to investigate the development of various cardiovascular diseases, how to set up and interpret biobank data. There is also a significant practical component where students will get hands-on bench work experience in the most common models used including quantitative live-cell assays, in situ hybridization, FISH staining, vascular function evaluation, and different sequencing data analysis.
Teaching and learning activities The course is a combination of theoretical and practical training, where lectures/group discussions and laboratory/practical demonstrations are integrated. Time is also allocated for discussing laboratory results and lectures.

Compulsory elements Full attendance is required to both theoretical and practical lessons in order to understand the course content and to pass the course. The students who have missed the group work sessions can book extra session time within four weeks to compensate for the absence.
Examination All the learning outcomes of the course have to be reached to pass the course. The final grade (pass or fail) will be based on:
- Summative assessment of the contributions during the discussions that are part of the course.
- Group presentation at the final seminar where different research topics regarding epigenetic modifications will be discussed, i.e. disease aspect to be investigated, and how the results of appropriate experiments are analyzed and interpreted.

Literature and other teaching material The publications listed below can be obtained via Pubmed search (https://www-ncbi-nlm-nih-gov.proxy.kib.ki.se/pubmed) and are recommended for students to read before the course.

1.Cell. 2019 Jun 27;178(1):242-260.e29. doi: 10.1016/j.cell.2019.05.010. Epub 2019 May 30.
PMID: 31155234
2.J Am Coll Cardiol. 2018 Apr 10;71(14):1584-1589. doi: 10.1016/j.jacc.2018.01.073.
PMID: 29622166
3. J Mol Cell Cardiol. 2019 Aug;133:148-163. doi: 10.1016/j.yjmcc.2019.06.010. Epub 2019 Jun 15.
PMID: 31211956
4. Circ Res. 2018 Apr 27;122(9):1221-1237. doi: 10.1161/CIRCRESAHA.118.310966.
PMID: 29700069
5.Adv Exp Med Biol. 2020;1229:121-132. doi: 10.1007/978-981-15-1671-9_6.
PMID: 32285408
Number of students 8 - 20
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) start date of doctoral studies (priority given to earlier start date)
More information The morning lectures will be held in room J3:20 Sven Ivar Seldingen, 3rd floor, Bioclinicum, Campus Solna. Afternoon in vivo, ex vivo, in vitro experiments and single cell and RNA-seq data analysis demo-workshop will be in different laboratories. There will be a special keynote seminar with two invited external speakers.
Additional course leader Zhichao Zhou
Latest course evaluation Course evaluation report
Course responsible Hong Jin
Institutionen för molekylär medicin och kirurgi

hong.jin@ki.se
Contact person Zhichao Zhou
Institutionen för medicin, Solna

zhichao.zhou@ki.se