Kurskatalog forskarutbildning - VT22

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Titel Flow cytometry: from theory to application
Kursnummer 3120
Program 1-Ingår i flera program
Språk Engelska
Antal högskolepoäng 1.5
Datum 2021-10-04 -- 2021-10-08
Kursansvarig institution Institutionen för medicin, Solna
Särskild behörighet
Kursens syfte The course aims to provide doctoral students with: 1) a systematic introduction of flow cytometry, 2) an overview of flow cytometric applications in biomedical research, and 3) a hands-on opportunity of flow cytometry lab training designed for various levels of flow cytometer users.
Kursens lärandemål Through this one-week course, the participants will acquire the knowledge of flow cytometry principles. They will know flow cytometric sample preparation and analysis, protocol set-up, major flow cytometric applications, as well as data interpretation and presentation.
Kursens innehåll The course covers basic knowledge and main applications of flow cytometry, and contains both lectures and laboratory/group work. Experts in different fields of flow cytometric application are invited to give the lectures.
The participants will be able to learn:
a) Principles of flow cytometry: working mechanisms of flow cytometer; molecular and cellular probes for flow cytometry; quality control; and safty procedures and intrument maintainance.
b) Basic skills: flow cytometric protocol design; sample handling and storage; data processing and analysis, and data presentation.
c) Major applications of flow cytometry in biomedical research: leukocyte phenotyping, endothelial cell analysis, stem cell analysis, apotosis, platelet measurement, cell function analysis (including membrane potential, oxidative metabolism, intracellular calcium mobilization, pH, and intracellular organelles), as well as nucleic acid analysis.
Arbetsformer The course includes 28 hr lectures and 12 hr hands-on lab work.
Obligatoriska moment The participants must attend hands-on lab sections during two afternoons. The students who have missed these sections can book extra lab section time within 4 weeks to compensate the absence.
Examination The exam consists of two parts: a 30 min written test on flow cytometric principles; and a 60 min hands-on lab work examining flow cytometric lab skills.
Kurslitteratur och övriga läromedel The course provides organized handouts from all lectures.
Antal studenter 20 - 32
Urval av studenter Selection will be based on: 1) The relevance of flow cytometry for the applicant’s project (according to written motivation) 2) Urgency of flow cytometry training for the applicant’s project (according to written motivation) 3) Previous application (priority given to those who have applied previously)
Övrig information Course will be given during week 40 (Oct 4-8, 2021; 9:00-16:30). Major part of the course will likely be organized virtually, i.e., lectures via Zoom. The organizers will try their best to implement half-day or one-day on-site lab sessions for the course. If on-site lectures are allowed and practical, a lecture hall will be booked T4:00 at Karolinska University Hospital-Solna. Lectures will be given by 14-15 lecturers, usually half of them from institutions outside KI, with two to three lecturers from abroad. All the lecturers are well-established experts in their lecture subjects of flow cytometric applications. The course has been given 1-2 times/year at KI for 20 years. It has been highly appreciated by the participants through the years, with a median general satisfaction score never below 8 (out of 9; referred to paper-based course evaluation between 2001-2013), and highly remarked with the electronic course evaluation (since 2014). The course is given jointly by the doctoral programmes Cardiovascular Research (CVR) and Allergy, immunology and inflammation (Aii). See: https://staff.ki.se/doctoral-programmes
Ytterligare kursledare
Senaste kursvärdering Kursvärderingsrapport
Kursansvarig Nailin Li
Institutionen för medicin, Solna
08-51773996
Nailin.Li@ki.se

Clinical Pharmacology Unit
Karolinska University Hospital-Solna
17176
Stockholm
Kontaktpersoner Nailin Li
Institutionen för medicin, Solna
08-51773996
Nailin.Li@ki.se

Clinical Pharmacology Unit
Karolinska University Hospital-Solna
17176
Stockholm