Course catalogue doctoral education - VT22

  • Application can be done between 2021-10-15 and 2021-11-15
Application closed
Title Human Cell Culture. Methods and Applications
Course number 3127
Programme Cell Biology and Genetics
Language English
Credits 1.5
Date 2021-10-04 -- 2021-10-08
Responsible KI department The institute of Environmental Medicine
Specific entry requirements
Purpose of the course The purpose of the course is to enable doctoral students to acquire state-of-the art knowledge and good understanding of human cell culture.
Intended learning outcomes After the course the students should be able to:
- Describe the theory and give examples of practical applications of human cell culture.
- Explain basic and state-of-the-art methods applied to cell cultures.
- Discuss possibilities and challenges in cell culture work.
Contents of the course Cell culture reflecting stem, transit amplifying, differentiated and terminally differentiated tissue states. Monolayer and organotypic culture involving one or more cell types. Applicability of cell cultures as alternatives to laboratory animal experiments. Mechanisms regulating cell growth and viability, differentiation and apoptosis. Assessment of cell transformation to immortal and malignant phenotypes. Isolation of specific cells, e.g., epithelial cells, characterisation of cultured cells. Handling and sterile techniques, choice of materials and media for cell culture, e.g., serum-dependent vs. serum-free culture conditions. Cell cloning and gene transfer. Practical handling of cultures: thawing/freezing, passage, expansion and long-term storage. Handling of normal and tumor tissue for optimizing obtainment of cultures. High-throughput screening technologies. Tissue engineering practices. Transcriptomics, proteomics and informatics methods for biomedical research with cell lines. Discussion of participants' own culture experience and problems.
Teaching and learning activities Interactive lectures, laboratory work, computer exercises and group discussions on pitfalls and possibilities with cell cultures.
Compulsory elements Participation in interactive lectures, group discussions, laboratory work and oral examination is compulsory. Absence from compulsory elements can compensated by participation at the next course occasion.
Examination Examination is in the form of a written assignment and oral presentation.
Literature and other teaching material Handouts are provided for all elements of the course.
Number of students 8 - 15
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) start date of doctoral studies (priority given to earlier start date)
More information
Additional course leader
Latest course evaluation Course evaluation report
Course responsible Penny Nymark
The institute of Environmental Medicine
Contact person Johanna Bergman
Institutet för miljömedicin

Nobels väg 13