Course catalogue doctoral education - VT22

  • Application can be done between 2021-10-15 and 2021-11-15
Application closed
Title Cryobiology in assisted reproductive technology
Course number 3089
Programme Development and regeneration (DevReg)
Language English
Credits 1.5
Date 2021-11-22 -- 2021-11-26
Responsible KI department Department of Biosciences and Nutrition
Specific entry requirements
Purpose of the course The aim of the course is to instruct the participants in human and mouse reproductive biology with focus on cryopreservation technology and embryo banking, as well as to give them practical experience through practical demonstrations in cryopreservation of gametes and embryos in IVF technology. To provide understanding of principles of cryobiology and components of culture systems used in assisted reproduction technology (ART). Thus the student can evaluate, troubleshoot and improve existing technology.
Intended learning outcomes The objectives of this course are that at the conclusion of this course students should have a good understanding of: Folliculogenesis and Fertilization; Preimplantation Embryology; sperm and oocytes retrieval; criteria of selected or scoring the gametes and zygotes for cryopreservation; liquid nitrogen handling; principles of cryobiology. Students should be aware of the general aspects and implication of the cryobiology research and the potentiality that this represents for clinical application; The principles of cryobiology; The Cryoprotectants additives and how they protect the cells by stabilizing intracellular proteins; The factors that affect cellular response to freezing; The different cryopreservation protocols and what is ongoing in this field; The cross-contamination of samples in liquid nitrogen; problems in achieving a good result of cryopreservation procedure; The possible epigenetic effects of the cryopreservation procedure; testicular and ovarian tissue cryopreservation procedures; Storage of the cryopreserved samples, the advantages of the cryopreservation and embryo bank. Finally the students will improve their capacity to produce coherent, logical and concise explanations of data and concepts - both written and oral, through consideration of the course material. Students will also develop their ability to criticize scientific literature related with cryopreservations technology and reproduction physiology in a constructive and informed fashion; Be aware of potential development of cryobiology and IVF in the future.
Contents of the course Contents
. Sperm-and ovogenesis and Hormonal function
. Main components of the culture system
. Physical-chemical properties of culture system
. Functional characteristics of different tissue culture incubators
. The influence of the laboratory and clinic environment on embryo culture
. The morphology of oocytes, zygotes and cleavage stage embryos as well as morulae and blastocysts
. Developmental milestones
. Biochemical tests for predicting developmental potential
. Possible effects of culture and frozen condition on epigenetics events
. Clinical aspects of frozen embryo transfer
. The potential of the different cryopreservation techniques in clinic application with focus on IVF
. General aspects and implication of the cryobiology in research
Teaching and learning activities Divided in lectures and laboratory demonstrations:
1. Embryology theory (lectures) 2.Culture system (lectures) 3. cryobiology (lectures) 4. Gametes and embryos quality evaluation (lectures) Lab.1. Laboratory demonstration in gametes and embryo retrieval; In-vitro fertilization and cryoprotectants additive; Handling with liquid nitrogen (lab). Lab.2. Evaluation of the quality of the fertilize oocytes and selection for freezing; performed cryopreservation on the selected samples (lab). Lab.3. Thawing frozen samples and quality assessment (lab). 6. Genetics and epigenetics aspects (lectures) 7. Introduction to the different cryopreservation procedures (lectures) 8. New trends in gametes and embryo cryopreservation (lectures) 9. Fertility preservation and challenge (lecture) 10. Future in human cryobiology and ethical aspects (lecture). Course test, evaluation, discussions, examination and closing of course.
Compulsory elements The laboratory demonstrations are obligatory; there will be lectures combined with laboratory demo, but if the student will be absent of one laboratory section, the student should present a literature work related to the subject of the missing activity.
Examination Examination on day 5, with individual or group presentations of the literature work related to the contents of the course, discussions and feedback. Every student will be individually assessed and the students has to show that all learning outcomes have been reached.
Literature and other teaching material Handbook of Human Oocyte Cryopreservation, Eleonora Porcu, Patrizia Ciotti, Stefano Venturoli. Cambridge University Press, 6 dic. 2012 - 151 pages
Gonadal Tissue Cryopreservation in Fertility PreservationEditors: Suzuki, Nao, Donnez, Jacques (Eds.) 2016
Cryopreservation: Modern Insights Hardcover, 31 Jan 2015 by Marianne Wilde(Editor)
Cryopreservation and Freeze-Drying Protocols, Editors: John G. Day and Glyn N. Stacey . ISBN: 978-1-58829-377-0 (Print) 978-1-59745-362-2 (Online)
Infertility; Julius Hreinsson, Lars Hamberger, Thorir Hardarson, Studentlitteratur, 2005
IVF LAB ""Laboratory aspects of in-vitro fertilization"" Editor: M. Bras, J.W. Lens, M. H. Piederiet, P.M. Rijinders, M. Verveld, G.H. Zeilmaker, 1996
Number of students 8 - 14
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date).
More information The course will be held at Karolinska Institutet, Department of Bioscience and Nutrition, NEO Huddinge.
Additional course leader
Latest course evaluation Course evaluation report
Course responsible Jose Inzunza
Department of Biosciences and Nutrition
08-585 850 93

Hälsovägen 7, Novum

141 86
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