Course catalogue doctoral education - VT21

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Title Basic Electron Microscopy for Cell Biologists
Course number 3219
Programme Cellbiologi och genetik
Language English
Credits 1.5
Date 2021-05-03 -- 2021-05-07
Responsible KI department Institutionen för cell- och molekylärbiologi
Specific entry requirements
Purpose of the course The students take this course in order to learn about the ways electron microscopy methods are used in the study of biological problems, and to gain knowledge in the theoretical basis of electron microscopy and some practical starting skills in electron microscopy methods.
Intended learning outcomes After the completed course, the students understand and can explain the theoretical basis of electron microscopy techniques, and explain how different kinds of biological research problems are studied using electron microscopy techniques. The students have basic knowledge on the application of different preparation methods, and can critically analyse and relate them to cell biological research questions. The students have basic (novice level) practical knowledge on the different practical preparation techniques.
Contents of the course The course introduces students to electron microscopy and the kind of biological problems such as different biological functions and structures that are studied using electron microscopy. The course includes training in basic methods used to do research on biological material such as subcellular structures. The course includes theoretical lectures, discussions and practical sessions on specimen preparation for conventional and cryo-transmission electron microscopy. Most important tissue processing methods for electron microscopy: fixation, dehydration, resin embedding, sectioning, negative staining of ultrathin sections. There are demonstrations on preprative methods in electron microscopy. Some Cryo-EM techniques will be demonstrated.
Teaching and learning activities The learning and teaching activities include talks (lectures), discussions, laboratory activities and demonstrations.
Compulsory elements The practical laboratories and demonstrations are obligatory. Absence from obligatory moments is regulated by instructions of the course leader.
Examination The outcomes are examined through an individual quiz with multiple choice questions, and a short individual written report/reflection on how the participants can employ EM techniques in their own research.
Literature and other teaching material Recent articles and different web resources.
Number of students 8 - 12
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) start date of doctoral studies (priority given to earlier start date)
More information The course will be held on the Karolinska Institutet's Solna campus. Lectures and final exam will be held in Biomedicum while practicals and demonstrations will be held in the KI 3D-EM facility (Nobels v. 12E). Designated time is 9:00 to 17:00 Monday to Friday including time for individual studies and group work. Assembly on the first day is in Biomedicum B0317 at 0900. The days typically consist of lectures before lunch and practicals/demonstrations in the afternoons. Practicals and demonstrations will include vitrification, cryo-confocal imaging for object identification, cryo-FIB-SEM preparation methods for transmission electron microscopy, cryo-volume imaging using FIB-SEM and cryo-transmission electron microscopy.
Additional course leader Sergej Masich
Latest course evaluation Not available
Course responsible Martin Hällberg
Institutionen för cell- och molekylärbiologi
Contact person Linda Lindell
Institutionen för cell- och molekylärbiologi
08-524 872 90

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