Course catalogue doctoral education - HT21

  • Application can be done between 2021-04-15 and 2021-05-17
Application closed
Title Human embryonic stem cells
Course number 2212
Programme Development and regeneration (DevReg)
Language English
Credits 1.5
Date 2021-05-17 -- 2021-05-21
Responsible KI department Department of Biosciences and Nutrition
Specific entry requirements
Purpose of the course The purpose of the course is to enable doctoral students to obtain a basic understanding of human reproductive biology with focus on human embryonic stem cell knowledge and cells replacement therapies and translational medicine. Experts in the field will provide a fresh overview of clinical and pre-clinical research aiming at development of novel treatment possibilities, but also discussing current limitations and general ethical aspects.
Finally the students will be enabled to improve their capacity to produce coherent, logical and concise explanations of data and concepts - both written and orally, through consideration of the course material.
Intended learning outcomes At the conclusion of this course students should be able to show a comprehensive view of:
- Pre implantation Embryology
- Derivation methods and culture conditions of hESCs
- Nutritional requirements of the blastocyst and stem cells
- Functional characteristics of different tissue culture incubators
- Characterization of the embryonic stem cells and the importance of the pluripotency of these cells and what is ongoing in this field
- Production of isogenics embryonic stem cells by somatic cell nuclear transfer (SCNT)
- The pluripotence induction of somatic cell by transduction (the iPS cells)
- Know the prospective possibilities of having a good culture system and be aware of potential development of hESC technology in the future.
- Be aware of the general aspects and implication of the stem cells research and the potentiality that these represent for clinical application.
Contents of the course 1. Embryology theory (lectures)
2. In vitro culture system (lectures)
3. Human Embryonic Stem cells culture (lectures)
4. Characterization of hESC (lectures)
5. Laboratory demonstrations
6. Genetics (lectures)
7. Introduction to different cell types (lectures)
8. Cell reprogramming (lectures)
9. Stem cells therapy and challenge today
10. Future in human embryonic stem cells (lecture)

Course test, evaluation, discussions, examination and closing of course.
Teaching and learning activities Lectures and laboratory demonstrations.
Compulsory elements The laboratory parts are obligatory. If absent at laboratory activity; student should present a literature work related with the subject of the missing activity
Examination Written individual examination
Literature and other teaching material Recommended literature:
The Global politics of human embryonic stem cell science,Regenerative medicine transition, By, Herbert Gottwels, Brain Salter and Catherine Waldby, Publisher:Palgrave Mac Milan, 2009.
Emerging technology platform for stem cells, edited by Uma lakshmipathy, Jonathan D. Chesnut.... Publisher: John Wiley & sons Ltd, 2009
Guidelines for human embryonic stem cell research, (Authors) Board of life sciences; National academy of sciences, Publisher: National academies press, 2005
Stem cell Research: Medical application & Ethical controversy, Joseph Panno, The New Biology, 2005
Human Embryology, William J. Larsen, Third Edition, Churchill Livinstone, 2001
Manipulating the mouse embryos ""Laboratory manual"" third edition Andras Nagy, Marina Gertsenstein, Kristina Vintersten, Richard Behringer, 2003
IVF LAB ""Laboratory aspects of in-vitro fertilization"" Editor: M. Bras, J.W. Lens, M. H. Piederiet, P.M. Rijinders, M. Verveld, G.H. Zeilmaker, 1996
Number of students 8 - 14
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information
Additional course leader The course will be held at Karolinska Institutet, Department of Biosciences and Nutrition, NEO Huddinge.
Latest course evaluation Course evaluation report
Course responsible Jose Inzunza
Department of Biosciences and Nutrition
08-585 850 93

Hälsovägen 7, Novum

141 86
Contact person Jose Inzunza
Institutionen för biovetenskaper och näringslära
08-585 850 93

Hälsovägen 7, Novum

141 86