Course catalogue doctoral education - VT21

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Title Experimental Models in Cardiovascular and Metabolism Research
Course number 5225
Programme Kardiovaskulär forskning
Language English
Credits 1.5
Notes A Laboratory Animal Science course.

Date 2020-10-19 -- 2020-10-23
Responsible KI department Institutionen för molekylär medicin och kirurgi
Specific entry requirements No prerequisite animal study knowledge is required because the teaching and practicing will be carried out on euthanatized animals and silicone pads.
Purpose of the course The purpose of the course is to enable the participants to obtain practical experience in ex vivo and in vivo models of cardiovascular and metabolism research as well as to discuss with fellow students and experts in the field the theory of the models and ethical aspects of translation research.
Intended learning outcomes At the end of the course the participant should be able to:
- Show an understanding of the main cardiovascular and metabolic disease models and their applications
- Comprehend the benefits and limitations of these models
- Learn how to breed and apply genetically modified animal models, as well as achieve and analyze data in the most optimal fashion
- Practically perform experiments using common current models for cardiovascular and metabolic diseases
Contents of the course The course is aimed at participants who are starting or have just started to use animal models in cardiovascular and metabolism research. The course will give theoretical knowledge about 1) Why and how to choose animal models to investigate relevant cardiovascular and metabolic diseases. 2) Current strategies on genetically modified animals including mice, rats and mini pigs. 3) How to collect in vivo and ex vivo data and analyze. There is also a practical component where students will get hands-on experience in the most common models used, as well as basic surgical technique practice on euthanatized mice and silicone pads instructed by experienced animal study surgeons.
Teaching and learning activities The course is partly theoretical, partly practical, where lectures/group discussions and laboratory demonstrations are integrated. Time is also allocated for discussing of laboratory results and lectures.
Compulsory elements Lectures/group discussions and laboratory demonstrations, as well as the examination, are compulsory. Absence of up to 10 percent in lectures, discussions and laboratory demonstrations can be compensated for in agreement with the course leader.
Examination All the learning outcomes of the course have to be reached to pass the course. The final grade (pass or fail) will be based on:
- formative and summative assessment of the contributions during the discussions that are part of the course
- individual presentation at a seminar where different aspects of an animal model will be discussed, i.e. disease aspect to be investigated, which model would suit best, and how are the results analyzed and interpreted
Literature and other teaching material Recommended literature:
Animal Models for the Study of Human Disease http://www.sciencedirect.com.proxy.kib.ki.se/science/book/9780124158948#ancp3
https://doi.org/10.1016/C2011-0-05225-0

Genetically modified pigs to model human diseases http://link.springer.com.proxy.kib.ki.se/article/10.1007/s13353-013-0182-9/fulltext.html

Inflammation and atherothrombosis in Clinical Immunology (Third Edition)
http://www.sciencedirect.com.proxy.kib.ki.se/science/article/pii/B9780323044042100685
https://doi-org.proxy.kib.ki.se/10.1016/B978-0-323-04404-2.10068-5

The Role of Animal Models in Elucidating the Etiology and Pathology of Abdominal Aortic Aneurysms: Development of a Novel Rupture Mechanism Model.Tanaka H. Ann Vasc Surg. 2020 Feb;63:382-390

Peripheral vascular atherosclerosis in a novel PCSK9 gain-of-function mutant Ossabaw miniature pig model. Hedayat AF.Transl Res. 2018

More model-specific related references will be included in the handouts by lecturers.
Number of students 8 - 12
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) start date of doctoral studies (priority given to earlier start date)
More information
Additional course leader Jiangning Yang
Latest course evaluation Not available
Course responsible Hong Jin
Institutionen för molekylär medicin och kirurgi

hong.jin@ki.se
Contact person Jiangning Yang
Institutionen för medicin, Solna

Jiangning.Yang@ki.se