Course catalogue doctoral education - HT20

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Title Phenotyping of genetically engineered mice
Course number 1974
Programme 0-Inte del av forskarutbildningsprogram
Language English
Credits 2.0
Notes A Laboratory Animal Science course.

Date 2019-11-12 -- 2019-11-21
Responsible KI department Komparativ medicin
Specific entry requirements Students need to have completed a laboratory animal science course on how to carry out scientific procedures on the appropriate animal species i.e. EU Function A or equivalent course.
Purpose of the course The course is aimed at doctoral students and postdocs who are in the initial phase of setting up animal experiments using mice. Researchers are introduced to the concept of integrative, comprehensive phenotype analysis with attention to international harmonization of experimental approach and reporting. Emphasis will be focused on morphological phenotype analysis in relation to age, development and in vivo physiological and behavioral parameters. Students will need to prepare oral presentations, which encourage them to reflect on the application of the course content in their own research area, and encourages them to discuss and explain their work with other participants.
Intended learning outcomes After completion of the course the students should be able to:
1) describe systematic and standardized phenotype analysis of transgenic mice and mice carrying targeted mutations;
2) present and review strategies for production and breeding of genetically engineered mice, with attention to accurate selection of controls;
3) practically handle techniques for analysis of general parameters, experiment termination and organ selection and sampling;
4) select more specialized approaches for evaluation of (neuro)behavioral, physiological and pathomorphological parameters.
Contents of the course The course provides instrumental knowledge for strategic select of endpoints and controls so as to maximize experimental outcome and rationalize the use of experimental animals. Attendees will be presented with theory on breeding, and schedules will be practiced with attention for selection of appropriate controls. Basic knowledge of mouse comparative biology and systematic evaluation of mouse phenotype will be presented during the course, and approaches for experiment termination and organ sampling (necropsy) will be discussed and practically trained. Specialized approaches for evaluation of (cardiovascular) physiology, (neuro) behavioral parameters, and non-invasive as well as post mortem morphological analysis will be presented.
Teaching and learning activities The course will consist of lectures, demonstrations and practical necropsy training. Lectures and demonstrations will be interactive with attention to specific research areas as requested by the participants. Participants will practice with breeding schedules for complex genetic modifications and prepare a presentation overviewing application of acquired insights to their area of research. The course is 5 days full-time. Presentations/practical exercises are during office hours, time will be provided to interact with team task-group members during office hours but the necessity for some homework is expected.
Compulsory elements All scheduled sessions (lectures, demonstrations, practical training and oral presentations/discussions) and active student participation are compulsory if the student is to be provided with certification of the successful completion of the course. Missed parts of the course as a consequence of a well-justified absence will need to be compensated after agreement with the course director e.g. with written assignment.
Examination To pass the course the student must actively participate in the lectures and group seminars, and pass the examinations:
1) Individual examination of multiple choice questions during the course;
2) Satisfactorily demonstrate a completed necropsy during the practical;
3) Presentation and discussion of group work summarizing the presented information as relevant for the area of research predominating in the group (3-4 participants per group).
A pass/fail criteria will be used as a global rate for this course.

Literature and other teaching material Virginia E Papaioannou and Richard R. Behringer. Mouse Phenotypes: A Handbook of Mutation Analysis. Cold Spring Harbor Laboratory Press 2004.

Recommended reading:
1. Lee M Silver. Mouse Genetics.
2. Margaret J. Cook. The Anatomy of the Laboratory Mouse.
3. Janet Rossant and Patrick PL Tam. Mouse Development: Patterning, Morphogenesis, and Organogenesis. Academic Press 2002.
4. P Popesko, V Rajtova and J Horak. A Colour Atlas Anatomy of Small Laboratory Animals: Volume Two: Rat, Mouse, Hamster. Academic Press. ISBN: 0-7020-2699-9
5. Hans Hedrich. The Laboratory Mouse (Handbook of Experimental Animals). Academic Press 2004. ISBN: 0123364256
6. Peggy Danneman, Mark Suckow, and Cory Brayton. The laboratory mouse. CRC Press 2012. ISBN: 9781439854211
7. Robert Maronpot. Pathology of the Mouse: Reference and Atlas. Cache River Press 1999. ISBN: 188989902X
8. Jacqueline N. Crawley. What's Wrong with My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice
9. Walter F. Loeb and Fred W. Quimby. Clinical Chemistry of Laboratory Animals
10. Piper Treuting and Suzanne Dintzis. Comparative Anatomy and Histology- a mouse and human atlas. Elsevier 2012. ISBN: 978-0-12-381361-9

Internet resources:

1. Mouse Phenome Database: Phenotypes and Biology of Genetically-Engineered Mice:
2. European Mouse Phenotyping Resource of Standardised Screens (EMPReSS):
3. Revised guidelines for organ sampling and trimming in rats and mice:
Number of students 8 - 16
Selection of students Applicants must have completed an appropriate course in laboratory animal science (e.g. to carry out scientific procedures on animals). If necessary, priority will be given to applicants using mouse models in their research project (explained according to a written motivation) and to applicants with an earlier registration date as doctoral students.
More information This course is a collaboration between the Unit for Education and Training in Laboratory Animal Science, Comparative Medicine, Karolinska Institutet, and the FENO (Morphological Phenotype Analysis) Core Facility at the Department of Laboratory Medicine, Karolinska University Hospital Huddinge. Course leaders are Assoc. Prof. Rafael Frias (Comparative Medicine) and Dr Raoul Kuiper (Laboratory Medicine). Invited speakers include both local and international experts in the topics. The course runs between November 12-21, 2019. Face-to-face lectures, hands-on exercises, oral presentations and group discussions will take place on site for 5 days within this time frame.
Additional course leader Rafael Frías | DVM, MSc (LAS), PhD, Assoc. Prof Unit for Education and Training in Laboratory Animal Science Comparative Medicine, Karolinska Institutet AND Raoul Kuiper, PhD DVM Dipl. ECVP pathology Department of Laboratory Medicine FENO, F52 Karolinska University Hospital Huddinge
Latest course evaluation Course evaluation report
Course responsible Rafael Frias
Komparativ medicin
Contact person Raoul Kuiper
Institutionen för laboratoriemedicin