Syllabus database for doctoral courses
-
Startpage
Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | Grundläggande elektronmikroskopi för cellbiologer |
|---|---|
| English title | Basic Electron Microscopy for Cell Biologists |
| Course number | 3219 |
| Credits | 1.5 |
| Responsible KI department | Institutionen för cell- och molekylärbiologi |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Committee for Doctoral Education |
| Established | 2022-09-14 |
| Purpose of the course | The students take this course in order to learn about the ways electron cryomicroscopy (Cryo-EM) methods are used in the study of biological problems, and to gain knowledge in the theoretical basis of electron microscopy and some practical starting skills in electron microscopy methods. |
| Intended learning outcomes | After the completed course, the students understand and can explain the theoretical basis of Cryo-EM techniques, and explain how different kinds of biological research problems are studied using these techniques. The students have basic knowledge on the application of different preparation methods, and can critically analyse and relate them to cell biological research questions. The students have basic (novice level) practical knowledge on the different practical preparation techniques. |
| Contents of the course | The course introduces students to cryo-EM and the kind of biological problems such as different biological functions and structures that are studied using electron microscopy. The course includes training in basic methods used to do research on biological material such as subcellular structures. The course includes theoretical lectures, discussions, a small literature project and practical sessions on specimen preparation for cryo-transmission electron microscopy. Most important sample preparation methods for cryo-electron microscopy: vitrification, cryo-confocal imaging for object identification, cryo-FIB-SEM preparation methods for transmission electron microscopy, cryo-volume imaging using FIB-SEM and cryo-transmission electron microscopy. There will be practicals on relevant methods in cryo-electron microscopy. |
| Teaching and learning activities | The learning and teaching activities include talks (lectures), discussions, laboratory activities and demonstrations. |
| Compulsory elements | The practical laboratories and demonstrations are obligatory. Absence from obligatory moments is regulated by instructions of the course leader. |
| Examination | The outcomes are examined through an individual quiz with multiple choice questions, and a short individual written report/reflection on how the participants can employ EM techniques in their own research. |
| Literature and other teaching material | Recent articles and different web resources will be distributed well in advance of the start of the course. | Course responsible |
Martin Hällberg Institutionen för cell- och molekylärbiologi Martin.Hallberg@ki.se |
| Contact person |
Linda Lindell Institutionen för cell- och molekylärbiologi 08-524 872 90 0703822909 linda.lindell@ki.se Solnavägen 9 171 77 Stockholm |
