Syllabus database for doctoral courses
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Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | Cell och genterapi |
|---|---|
| English title | Cell and gene therapy |
| Course number | 2444 |
| Credits | 1.5 |
| Responsible KI department | Institutionen för medicin, Huddinge |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Board of Doctoral Education |
| Established | 2011-03-28 |
| Purpose of the course | |
| Intended learning outcomes | After the course, the student should be able to; 1. Explain mechanisms that are involved in viral gene integration in mammalian cells. 2. Apply their knowledge in molecular biology to genetic modification of mammalian cells. This means they should be able to develop cloning strategies for your gene of interest into a viral vector and propose a strategy for in vivo genetic manipulation. 3. Apply their knowledge in single gene disorders and develop treatment schemes for patients with these disorders using gene therapy. |
| Contents of the course | The first part of the course will cover the basic aspects of gene regulation, cell culture, and gene transfer methods. In addition, the recent advances in vector development will be discussed. The second part of the course will deal with human studies, with special emphasis on the hematology field. |
| Teaching and learning activities | The pedagogic learning activities on the course consist of lectures, group discussions, student presentations and laboratory practices. |
| Compulsory elements | All practical sessions, all group works and some of the lectures are compulsory. Missed parts can be completed after agreement with the course organizers. |
| Examination | The students are examined individually with a quiz or a written essay. The final course examination is a project presentation in groups. |
| Literature and other teaching material | Books for reference (Freely available via google books): B. Kelly. Gene therapy¿. (2007) Panno. Gene therapy: treating disease by repairing genes¿. (2004) M. Rubanyi and Ylä-Herttuala. Human gene therapy: current opportunities and future trends¿. (2003) A. Machida. Viral vectors for gene therapy: methods and protocols¿. (2003) Articles: Baum et al. Chance or necessity? Insertional mutagenesis in gene therapy and its consequences. Molecular Therapy (2004) vol. 9 (1) pp. 5-13 Schumacher. T-cell-receptor gene therapy. Nat Rev Immunol (2002) vol. 2 (7) pp. 512-9 Cavazzana-Calvo et al. The future of gene therapy. Nature (2004) vol. 427 (6977) pp. 779-81 Williams and Baum. Medicine. Gene therapy--new challenges ahead. Science (2003) vol. 302 (5644) pp. 400-1 Aiuti et al. Gene therapy for adenosine deaminase deficiency. Current opinion in allergy and clinical immunology (2003) vol. 3 (6) pp. 461-6 Baum et al. Mutagenesis and oncogenesis by chromosomal insertion of gene transfer vectors. Hum Gene Ther (2006) vol. 17 (3) pp. 253-63 Bollard et al. Gene-marking studies of hematopoietic cells. Int J Hematol (2001) vol. 73 (1) pp. 14-22 Braun et al. Gene therapy strategies for leukemia. Molecular medicine today (1997) vol. 3 (1) pp. 39-46 Burger et al. Cellular engineering of HSV-tk transduced, expanded T lymphocytes for graft-versus-host disease management. Acta Haematol (2003) vol. 110 (2-3) pp. 121-31 Ivanov et al. Towards immunogene therapy of hematological malignancies. Exp Hematol (2006) vol. 34 (3) pp. 251-63 Martin and Lemoine. Direct cell killing by suicide genes. Cancer Metastasis Rev (1996) vol. 15 (3) pp. 301-16 Mesnil and Yamasaki. Bystander effect in herpes simplex virus-thymidine kinase/ganciclovir cancer gene therapy: role of gap-junctional intercellular communication. Cancer Res (2000) vol. 60 (15) pp. 3989-99 Robinet et al. Improving the ex vivo retroviral-mediated suicide-gene transfer process in T lymphocytes to preserve immune function. Cytotherapy (2005) vol. 7 (2) pp. 150-7 Seth. Vector-mediated cancer gene therapy: an overview. Cancer Biol Ther (2005) vol. 4 (5) pp. 512-7 Yazawa et al. Current progress in suicide gene therapy for cancer. World journal of surgery (2002) vol. 26 (7) pp. 783-9 Sutlu and Alici. Natural killer cell-based immunotherapy in cancer: current insights and future prospects. Journal of Internal Medicine (2009) vol. 266 (2) pp. 154-81 Barros Costa. Review Article: Targeted Therapy: Comprehensive Review. American Journal of Hospice and Palliative Medicine (2009) vol. 26 (2) pp. 137-146 |
Course responsible |
Evren Alici Institutionen för medicin, Huddinge 073-6261412 Evren.Alici@ki.se |
| Contact person |
Tolga Sutlu Institutionen för medicin, Huddinge 585887773 0762720267 tolga.sutlu@ki.se |
