Syllabus database for doctoral courses
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Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | Kalciumsignalering |
|---|---|
| English title | Calcium signaling |
| Course number | 2464 |
| Credits | 3.0 |
| Responsible KI department | Institutionen för klinisk forskning och utbildning, Södersjukhuset |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Board of Doctoral Education |
| Established | 2010-08-26 |
| Purpose of the course | |
| Intended learning outcomes | At the end of the course the participants will understand the fundamental molecular proceses involved in maintaining intracellular Ca2+ homeostasis and their roles in the generation and decoding of Ca2+- and phospholipids-mediated signals. They will be able to analyse and interpret the existing literatures critically, and address new question in this area of research. They will understand the principles of common methods including those of microscope based fluorometry in living cells, confocal and multiphoton imaging and electrophysiology. Participants will see actual demonstration of the methods used for measurement of intracellular free Ca2+ concentration by microfluorometry and confocal microscopy. After completion of the course, participants will be able to come up with new ideas, generate new hypotheses and identify approaches to test them by using diverse methods and approaches. |
| Contents of the course | 1. Phospholipase C and inositol 1,4,5 trisphosphate-mediated signaling. 2. Identity and roles of molecular players involved in Ca2+ and phospholipid mediated signaling. 3. Preparation of Ca2+ buffers. 4. Methods used in the study of calcium and phospholipid signaling including fluorescent techniques, electrphysiology and imaging techniques. 5. regulation of ion channels involved in Ca2+ signaling including voltage sensitive channels, transient receptor potential channels, store-operated channels and intracellular Ca2+ channels. 6. roles of Ca2+ and phospholipid mediated signaling in cellular processes including in secretion and apoptosis. 7. Mechanism of generation and decoding of Ca2+ signals. 8. spatial and temporal aspects of Ca2+ signaling. 9. How to pick research problems in the areas of Ca2+ and phospholipid signaling and how to approach them. |
| Teaching and learning activities | This course will follow the classical seven steps approach of problem-based-learning (PBL). Emphasis will be on self-directed learning through problem-solving in small groups rather than on cathedral lectures. However, generous small group interactive lectures by resource personnel and selected practical sessions will be provided. Participants will work on five given problems, in groups of about nine participants, under supervision of two trained facilitators who will be available during all of the sessions. Participants will be provided with an outline of the objectives, areas expected to cover, and reprints of selected learning materials. In practical sessions, participants will observe the use of fura-2-based microfluorometry and confocal microscopy for measurement of cytoplasmic free Ca2+ concentration. |
| Compulsory elements | Submission of a short written report is essential for obtaining a pass grade. The supervisors guides in writing this report. |
| Examination | 1. Observation by the facilitators of the participants role in the learning processes during group activitites. 2. Presentation of assignment in group-seminar. 3. Individual written reports. |
| Literature and other teaching material | 1. Calcium the molecular basis of calcium action in biology and medicine. edited by Roland Pochet ; co-edited by Rosario Donato. Kluwer academic publishers. 2000 2. Calcium signaling protocols. Methods in molecular biology. Edited by D. G. Lambert. Humana press. 2006. 3. M. Vig and J. P. Kinet. Calcium signaling in immune cells. Nat Immunol 10 (1):21-27, 2009. 4. K. Mikoshiba, C. Hisatsune, A. Futatsugi, A. Mizutani, T. Nakamura, and K. Miyachi. The role of Ca2+ signaling in cell function with special reference to exocrine secretion. Cornea 27 Suppl 1:S3-S8, 2008. 5. M. Oh-hora and A. Rao. Calcium signaling in lymphocytes. Curr.Opin.Immunol 20 (3):250-258, 2008. |
Course responsible |
Shahidul Islam Institutionen för klinisk forskning och utbildning, Södersjukhuset 086163950 0702593446 Shahidul.Islam@ki.se |
| Contact person |
