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Swedish title Mikroskopi av levande celler
English title Live cell imaging
Course number 2283
Credits 3.0
Responsible KI department Institutionen för cell- och molekylärbiologi
Specific entry requirements none
Grading Passed /Not passed
Established by The Board of Postgraduate Education
Established 2009-03-10
Purpose of the course
Intended learning outcomes After passing the completed course, the students will be familiar with the basic principles of fluorescence microscopy and diverse live cell imaging techniques. They will be competent in independently designing and performing live cell imaging experiments and understand the possibilities/limitations of different live cell imaging techniques.
Contents of the course Basic principles of fluorescence microscopy and live cell imaging.
Confocal Laser Scanning Microscopy
Deconvolution fluorescence Microscopy
Basic principles of advanced fluorescence techniques.
Design and perform live cell imaging experiments.
Data analysis and presentation.
Teaching and learning activities The pedagogic learning activities on the course consist of lectures, research seminars, laboratory work (imaging), group discussions, data processing and student presentations.
Compulsory elements All activities (lectures, research seminars, laboratory work, group discussions, data processing and student presentations) are compulsory. If students are unable to attend they have to discuss with course organizer how this can be compensated. Compensation of the laboratory work is not possible.
Examination The students are examinated through 1) an individually written assigment (case study), 2) a practical session and 3) a poster presentation.
Literature and other teaching material A basic compendium about the fluorescence microscopy, the different fluorescence techniques in live cell imaging.

Recent articles in which live cell imaging and the different fluorescence techniques are applied.
Course responsible Matti Nikkola
Institutionen för cell- och molekylärbiologi


Matti.Nikkola@ki.se
Contact person