Syllabus database for doctoral courses
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Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | Odling av celler från människa. Metoder och tillämpningar |
|---|---|
| English title | Human Cell Culture. Methods and Applications |
| Course number | 3127 |
| Credits | 1.5 |
| Responsible KI department | Institutet för miljömedicin |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Board of Doctoral Education |
| Established | 2018-08-16 |
| Purpose of the course | The purpose of the course is to enable doctoral students to acquire state-of-the art knowledge and good understanding of human cell culture. |
| Intended learning outcomes | After the course the students should be able to:
- Describe the theory and give examples of practical applications of human cell culture. - Explain basic and state-of-the-art methods applied to cell cultures. - Discuss possibilities and challenges in cell culture work. |
| Contents of the course | Cell culture reflecting stem, transit amplifying, differentiated and terminally differentiated tissue states. Monolayer and organotypic culture involving one or more cell types. Applicability of cell cultures as alternatives to laboratory animal experiments. Mechanisms regulating cell growth and viability, differentiation and apoptosis. Assessment of cell transformation to immortal and malignant phenotypes. Isolation of specific cells, e.g., epithelial cells, characterisation of cultured cells. Handling and sterile techniques, choice of materials and media for cell culture, e.g., serum-dependent vs. serum-free culture conditions. Cell cloning and gene transfer. Practical handling of cultures: thawing/freezing, passage, expansion and long-term storage. Handling of normal and tumor tissue for optimizing obtainment of cultures. High-throughput screening technologies. Tissue engineering practices. Transcriptomics, proteomics and informatics methods for biomedical research with cell lines. Discussion of participants' own culture experience and problems. |
| Teaching and learning activities | Interactive lectures, laboratory work, computer exercises and group discussions on pitfalls and possibilities with cell cultures. |
| Compulsory elements | Participation in interactive lectures, group discussions, laboratory work and oral examination is compulsory. Absence from compulsory elements can compensated by participation at the next course occasion. |
| Examination | Examination is in the form of a written assignment and oral presentation. |
| Literature and other teaching material | Handouts are provided for all elements of the course. | Course responsible |
Penny Nymark Institutet för miljömedicin penny.nymark@ki.se |
| Contact person |
Johanna Bergman Institutet för miljömedicin johanna.bergman@ki.se Nobels väg 13 17177 Stockholm Penny Nymark Institutet för miljömedicin penny.nymark@ki.se |
