Syllabus database for doctoral courses
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Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | Flödescytometri: från teori till praktik |
|---|---|
| English title | Flow cytometry: from theory to application |
| Course number | 3120 |
| Credits | 1.5 |
| Responsible KI department | Institutionen för medicin, Solna |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Board of Doctoral Education |
| Established | 2018-08-13 |
| Purpose of the course | The course aims to provide doctoral students with: 1) a systematic introduction of flow cytometry, 2) an overview of flow cytometric applications in biomedical research, and 3) a hands-on opportunity of flow cytometry lab training designed for various levels of flow cytometer users. |
| Intended learning outcomes | Through this one-week course, the participants will acquire the knowledge of flow cytometry principles. They will know flow cytometric sample preparation and analysis, protocol set-up, major flow cytometric applications, as well as data interpretation and presentation. |
| Contents of the course | The course covers basic knowledge and main applications of flow cytometry, and contains both lectures and laboratory/group work. Experts in different fields of flow cytometric application are invited to give the lectures. The participants will be able to learn: a) Principles of flow cytometry: working mechanisms of flow cytometer; molecular and cellular probes for flow cytometry; quality control; and safty procedures and intrument maintainance. b) Basic skills: flow cytometric protocol design; sample handling and storage; data processing and analysis, and data presentation. c) Major applications of flow cytometry in biomedical research: leukocyte phenotyping, endothelial cell analysis, stem cell analysis, apotosis, platelet measurement, cell function analysis (including membrane potential, oxidative metabolism, intracellular calcium mobilization, pH, and intracellular organelles), as well as nucleic acid analysis. |
| Teaching and learning activities | The course includes 28 hr lectures and 12 hr hands-on lab work. |
| Compulsory elements | The participants must attend hands-on lab sections during two afternoons. The students who have missed these sections can book extra lab section time within 4 weeks to compensate the absence. |
| Examination | The exam consists of two parts: a 30 min written test on flow cytometric principles; and a 60 min hands-on lab work examining flow cytometric lab skills. |
| Literature and other teaching material | The course provides organized handouts from all lectures. |
Course responsible |
Nailin Li Institutionen för medicin, Solna 08-51773996 073-6172673 Nailin.Li@ki.se Clinical Pharmacology Unit Karolinska University Hospital-Solna 17176 Stockholm |
| Contact person |
Nailin Li Institutionen för medicin, Solna 08-51773996 073-6172673 Nailin.Li@ki.se Clinical Pharmacology Unit Karolinska University Hospital-Solna 17176 Stockholm |
