Syllabus database for doctoral courses
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Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | In situ hybridisering: teori och praktik |
|---|---|
| English title | In situ hybridization: theory and practice |
| Course number | 2872 |
| Credits | 1.5 |
| Responsible KI department | Institutionen för medicin, Solna |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Board of Doctoral Education |
| Established | 2017-03-21 |
| Purpose of the course | In situ hybridization is a technique widely-used in many research fields, but it is challenging to set up. The purpose of this course is to enable the students to master this technique and be able to apply this tool in their own research projects. |
| Intended learning outcomes | At the end of the course the student should be able: 1. to describe the theoretical background behind in situ hybridization techniques. Discuss pros and cons in relation to the biological problem studied. 2. to design and setup in situ experiment based on her/his own research project, including optimization of conditions of the key steps during the experiment. 3. to perform and document experimental work in accordance with Good Laboratory Practice (GLP) guidelines. 4. to communicate the results in writing and orally. |
| Contents of the course | The course will cover the basics about theory and practice of in situ hybridization techniques. The focus will be on demonstrating and explaining current in situ hybridization techniques to detect RNAs (microRNA, long-non-coding RNA and mRNA) and DNA molecules in tissues / cells. The participant will get hand-on practice on in situ hybridization technique. |
| Teaching and learning activities | The course will start with introductory lectures, in which the invited technique experts will talk about the theoretical background and the cutting-edge progress about in situ hybridization techniques. The course participants will have a chance to perform an in situ hybridization experiment under supervision to detect RNAs in tissues. The course will end with an interactive exercise, discussing the theory and the lab data in addition to the application of in situ hybridization technique in the participants' own research projects. |
| Compulsory elements | All parts are mandatory. Absence from a lecture or discussion may be compensated by a written assignment, on agreement with the course leader. |
| Examination | Interactive exercise discussing how to apply in situ hybridization techniques in the participants' own research projects taking the theoretical aspects regarding in situ hybridization into account. Every student will be individually assessed. |
| Literature and other teaching material | Relevant publications covering the topic will be handed out by lecturers. | Course responsible |
Ning Xu Institutionen för medicin, Solna ning.xu@ki.se |
| Contact person |
