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Swedish title Cellulära avbildningstekniker och confokalmikroskopi
English title Cellular Imaging and Confocal Techniques
Course number 1725
Credits 3.0
Responsible KI department Institutionen för neurovetenskap
Specific entry requirements
Grading Passed /Not passed
Established by The Board of Postgraduate Education
Established 2007-11-08
Purpose of the course
Intended learning outcomes Knowledge and understanding:
After completion of the course, the graduate student should be able to 1) account for the different microscopy techniques which are being used for imaging of different types of cells and their properties, 2) account for the theoretical bases of different imaging techniques, 3) describe the principles to follow in selecting the suitable imaging technique for a particular scientific problem.
Skills:
The graduate student should be able to 1) describe the principles of operation of different microscopy systems, 2) independently select and apply a suitable imaging technique for a particular preparation, 3) describe the principles to follow in optimization and problem-solving in the use of different imaging techniques.
Professional attitudes:
The graduate student should be able to 1) evaluate the suitability of different imaging techniques for different types of preparations and scientific problems, 2) evaluate how different imaging techniques have been utilized in scientific articles, 3) critically consider pros and cons of different imaging techniques, 4) summarize and communicate his/her knowledge and understanding to other users of different microscopy systems.
Contents of the course Different methods for microscopic imaging, with an emphasis on fluorescence and confocal techniques, will be considered in detail. Techniques for imaging of fixed preparations, as well as live cell imaging of dynamic processes, will also be covered.
Teaching and learning activities The course duration is two weeks. First week (full-time, scheduled): Morning lectures (4 hours/day); afternoons - project work in groups with practical exercises and demonstrations on different imaging systems (4 hours/day; 4-5 systems to be used in parallel), seminars. Second week: Group work with project compilation and presentations.
Examination Group presentations of project work; individual, written exam.
Literature and other teaching material "Handbook of Biological Confocal Microscopy", 3rd edition, J.B. Pawley (ed), Springer-Verlag, 2006. Hand-outs and scientific articles distributed during the course.
Compulsory elements The compulsory parts of the course are the practical exercises and project presentations, and a written exam. To compensate for absence from a compulsory part the graduate student will have the opportunity to complement the project work, and to write an extra exam.
Course responsible Peter Wallén
Institutionen för neurovetenskap
0852486913

Peter.Wallen@ki.se

Retzius väg 8, A3-5

171 77
Stockholm
Contact person Per Uhlen
Institutionen för medicinsk biokemi och biofysik
0852487677

Per.Uhlen@ki.se