Syllabus database for doctoral courses
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Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | DNA skador och genomisk integritet |
|---|---|
| English title | DNA damage and maintenance of genome integrity |
| Course number | 2859 |
| Credits | 1.5 |
| Responsible KI department | Institutet för miljömedicin |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Board of Doctoral Education |
| Established | 2015-03-06 |
| Purpose of the course | |
| Intended learning outcomes | After the course, the student shall be able to: Regarding knowledge and understanding, - describe how exposure to various environmental agents and anti-cancer drugs can lead to modifications of DNA - account for various types of DNA damage and how they can be analyzed, as well as the molecular mechanisms by which cells detect, signal, and repair DNA damage to maintain the integrity of the genome - describe epigenetic mechanisms in relation to genome instability - account for the connections between DNA damage/DNA repair capacity and human disease, particularly cancer Regarding skills and ability, - plan and perform one established method used for studying genotoxic effects such as comet assay, micronuclei assay or gamma-H2AX-staining Regarding judgment and approach, - evaluate their own results and critically assess research papers relevant to the field of DNA damage, DNA repair and genome instability. |
| Contents of the course | Drugs and many environmental pollutants such as airborne particles, hydrocarbons, metals, manufactured nanoparticles as well as different mixtures may affect the genome integrity by causing changes in the genome or epigenome. Effective maintenance and interpretation of the genome is essential for normal cell division, tissue homeostasis, and cellular and organismal fitness. DNA damage and errors in DNA replication, recombination and repair, and epigenetic mechanisms all contribute to genomic instability and tumor progression. The course focuses on the formation of DNA damage as well as molecular mechanisms devoted to the preservation of genome integrity in eukaryotic cells. Topics include DNA damage formation and analysis, DNA damage recognition and repair pathways, cell cycle checkpoint mechanisms, and DNA repair as target for therapeutic intervention. The course emphasizes the relevance of these mechanisms in human disease development. The course also includes a practical part in which the students perform one selected assay for assessing DNA damage following cellular exposure to a substance of interest for the student. The course builds on a strong core of researchers with expertise in multiple aspects of genome biology across a broad range of research areas including Toxicology, Cancer biology, and Molecular Biology research. |
| Teaching and learning activities | The teaching includes lectures, seminars, laboratory sessions, oral presentations and group assignments. |
| Compulsory elements | Participation in the laboratory sessions is mandatory. Absence can be compensated with an individual task. |
| Examination | The students will present in an oral form their laboratory group work and demonstrate their skills in discussing their own work and the work presented by their fellow students. |
| Literature and other teaching material | Course responsible |
Kristian Dreij Institutet för miljömedicin 08-524 875 66 Kristian.Dreij@ki.se |
| Contact person |
Johanna Bergman Institutet för miljömedicin johanna.bergman@ki.se Nobels väg 13 17177 Stockholm |
