Syllabus database for doctoral courses
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Syllabus database for doctoral courses
SYLLABI FOR DOCTORAL COURSES
| Swedish title | Nyutvecklade fluorescens-baserade metoder inom immunologi |
|---|---|
| English title | Novel fluorescence-based methods in immunology |
| Course number | 2807 |
| Credits | 2.0 |
| Responsible KI department | Institutionen för mikrobiologi, tumör- och cellbiologi |
| Specific entry requirements | |
| Grading | Passed /Not passed |
| Established by | The Board of Doctoral Education |
| Established | 2014-09-09 |
| Purpose of the course | |
| Intended learning outcomes | At the end of the course, the students will be able to give an account of available state-of-the art fluorescence-based techniques to investigate different research questions within immunology. This includes an ability to compare and discuss the relative advantages and disadvantages of the different techniques, and in which situations the use of each one of them is appropriate. The students should be able to relate these limitations and benefits to their own research project. They should also be able to formulate and ask relevant questions about the different techniques to experts of the respective techniques. |
| Contents of the course | Novel ultra-sensitive fluorescence-based methods, that are now widely introduced, can provide pivotal information about both spatial distribution and temporary dynamics at all scales, from whole organisms to the molecular level. The course will introduce different state-of-the-art fluorescence-based techniques relevant to immunological research. These include techniques addressing spatial and temporal dynamics at the cellular and molecular level. At the cellular level, techniques to study cell migration in vitro and in vivo. At the molecular level, Fluorescence Correlation Spectroscopy (FCS) to measure diffusion and quantitate concentrations of molecules in live cells; Förster Resonance Energy Transfer (FRET) to measure the interaction between two molecules and conformational states; and super-resolution microscopy to measure localization of proteins down to single molecule level. |
| Teaching and learning activities | The course consists of three parts. The first two days will be dedicated to participation in a workshop on Novel Fluorescence Methods in Immunology. To facilitate interaction and to use the speaker's knowledge at the best, students will be asked to prepare by reading review articles about the techniques. The first day of the workshop will be technique oriented, with speakers introducing the different techniques. The second day invited speakers will talk about how they apply these techniques within immunology. The students will be involved in active interaction with both the other course participants and the speakers, through e.g. a poster session and panel discussions, directly targeting the students. On the third day, students will be divided in groups which will be given lab demonstrations of some of the techniques on a rolling schedule. In the third part of the course the students will produce a tentative research proposal each, based on one or two of the involved techniques. The students should motivate the choice of technique, and why the other techniques are not suitable. Students will be given opportunity for guidance in this process. |
| Compulsory elements | Active participation in all sections during the first three days is mandatory. Absence is generally not accepted, but if there are extraordinary circumstances a tailor-made session could be agreed with the course organizers, followed by an extra written examination, for maximum one day of absence. |
| Examination | The examination will consist of three parts. Firstly, the students will be asked to prepare and present a poster about their own research. During the poster session the students' ability to relate the usefulness of fluorescence-based techniques for their own research will be evaluated. Secondly, active participation e.g. by asking questions to the presenting researchers at the workshop will be required. This will relate to the goal of being able to compare the usefulness of different techniques, and the ability to ask relevant questions to experts within the field. Thirdly, the research proposal formulated during day 4-5 should be handed in as a report, 1-2 A4 pages. In the written report, the ability to give an account of the available state-of-the art fluorescence-based techniques, the ability to compare and discuss the relative advantages and disadvantages of the different techniques, and judge in which situations each is appropriate, will be evaluated. |
| Literature and other teaching material | Single-cell technologies for monitoring immune systems Nat Immunol. 2014 Feb;15(2):128-35. doi: 10.1038/ni.2796. A Decade of Imaging Cellular Motility and Interaction Dynamics in the Immune System Science 2012;336(6089):1676-1681 doi: 10.1126/science.1221063 http://www.sciencemag.org/content/336/6089/1676.full A guide to super-resolution fluorescence microscopy. J Cell Biol. 2010 Jul 26;190(2):165-75. doi: 10.1083/jcb.201002018. http://jcb.rupress.org/content/190/2/165.long The F-techniques: advances in receptor protein studies. 2008; 19(5):181-90. doi:10.1016/j.tem.2008.02.004 http://www.sciencedirect.com/science/article/pii/S1043276008000568 An introduction to fluorescence imaging techniques geared towards biosensor applications. Methods Mol Biol. 2014;1071:17-28. doi: 10.1007/978-1-62703-622-1_2. http://link.springer.com/protocol/10.1007/978-1-62703-622-1_2/fulltext.html#Sec00022 |
Course responsible |
Sofia Johansson Institutionen för mikrobiologi, tumör- och cellbiologi 08-52486470 sofia.e.johansson@ki.se Nobels väg 16 17177 Stockholm |
| Contact person |
