Course catalogue doctoral education - VT24
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| Title | Molecular localisation in cells using fluorescence microscopy |
|---|---|
| Course number | 2356 |
| Programme | Cellbiologi och genetik |
| Language | English |
| Credits | 3.0 |
| Date | 2010-09-27 -- 2010-10-08 | Responsible KI department | Institutionen för cell- och molekylärbiologi |
| Specific entry requirements | |
| Intended learning outcomes | After the finished course, the students will be able to describe the basics of the technology behind fluorescence and confocal microscopy, and the most common applications of these technologies in cell biology. |
| Contents of the course | The course contains both theoretical and practical modules or demonstrations focusing on the different techniques used for investigating molecular localisation in cells. These include different types of microscopes, sample preparation, immunostaining and visualization, and data analysis. |
| Teaching and learning activities | The learning forms include cathedral lectures, discussions, presentations, laboratory practicals and demonstrations. |
| Compulsory elements | The laboratory practicals and demonstrations are obligatory. Compensation with additional individual projects according to the instructions of the course director. |
| Examination | Examination reflecting the learning outcomes of the course is groupwise and individually with group projects and demonstrations. |
| Literature and other teaching material | Antibodies: A laboratory Manual; Ed Harlow, David Lane; Cold Spring Harbor Laboratory, 1988. Nonradioactive In Situ Hybridization Application Manual (Roche) - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/InSi_toc.htm Green Fluorescent Protein; Conn, P. M., Ed.; Academic Press: San Diego, 1999; Vol. 302 Green Fluorescent Protein: Methods in Cell Biology; Sullivan, K. F., Kay, S. A., Eds.; Academic Press: San Diego, 1998; Vol. 58. |
| Number of students | 8 - 15 |
| Selection of students | The selection is based on the relevance of the course content to the doctoral project and the personal motivation written by the applicant. |
| More information | The course lectures include: Fluorescence Microscopy: Introduction anddescription of principles; Specimen preparation; Applications; Confocal Microscopy in Biomedical Research: Different types of confocal microscopes and their uses; imaging live animals such as mice; improved resolution for examining movement of organelles and molecules in living cells; Colocalization and Interaction of Proteins: Differences between colocalization and interaction; Importance ofpreventing emission and excitation crosstalk; Using FRET (Fluorescence Resonance Energy Transfer) to show protein-proteininteractions; Live Cell microscopy to study cellular dynamics at the molecular level; Using time series to look at translocation of proteins Laboratory topics include: Different protocols for cell fixation andpermeabilization; Immunostaining of endogenous proteins andorganelles; Expression and visualization of GFP-tagged proteins; RNAvisualization by fluorescence in situ hybridization (FISH); Sample preparation for fluorescence microscopy; Analysis by light microscopy;Demonstration of data acquisition by confocal microscopy. The target group of the course is doctoral students using cell and molecular biology methods in their research. Students can bring their own antibodies and/or plasmid vectors for mammalian expression to study their own protein of interest. The course is full time and it is organized at the Department of Cell and Molecular Biology. The course is a collaboration between the doctoral course program in Cell Biology and Genetics and the DBRM Research school. The course web pages can be found at the DBRM Research school pages at www.dbrm.se. |
| Additional course leader | The course director is Dr. Piergiorgio Percipalle, email piergiorgio.percipalle@ki.se and the course co-director is Dr. Emilie Louvet. |
| Latest course evaluation | Not available |
| Course responsible |
Matti Nikkola Institutionen för cell- och molekylärbiologi Matti.Nikkola@ki.se |
| Contact person |
Inger Westin Institutionen för cell- och molekylärbiologi 0852487371 Inger.Westin@ki.se |
