Course catalogue doctoral education - VT20

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Title Experimental techniques in study of metabolic and endocrine disorders
Course number 3121
Programme Metabolism och endokrinologi
Language English
Credits 1.5
Date 2019-11-25 -- 2019-11-29
Responsible KI department Institutionen för molekylär medicin och kirurgi
Specific entry requirements
Purpose of the course This course will enable the doctoral student to acquire the necessary knowledge to address experimentally key points of metabolic characterization of experimental models in diabetes research.
Intended learning outcomes After the course the students will be able i) to measure glucose transport in isolated rodent skeletal muscle; ii) to measure lipolysis in isolated adipocytes; iii) to dissect out mouse pancreatic islets and measure the insulin release; iv) to judge and analyze obtained data.
The students will also be able to describe the possibilities and limitations of the above techniques.
Contents of the course The course is laboratory based, aiming to give all participants hands on experience with isolation of pancreatic islets, skeletal muscle and adipose tissue. Techniques for measurement of glucose transport in isolated rodent skeletal muscle, of lipolysis in isolated adipocytes, and for studying insulin release from pancreatic islets will be covered. Theoretical and practical considerations will be presented and discussed.
Teaching and learning activities The course meets for five days full time, including three full day laboratory practical sessions. The first day will consist of several lectures to give a background to the metabolic questions which will be addressed in the practical part of the course. Our aim is to provide the student with a hands on experience of each technique covered. In order to achieve this, for the laboratory work the course participants will be subdivided into smaller groups.
Compulsory elements All three laboratory tasks are compulsory; as an exception, a written task could be given to a participant to compensate absence.
Examination Summative assessment of the laboratory notebook notes from each student, of a short oral presentation of the project work, of the discussions with the course leader and the other students, and of a short summary of the project work by each group.
Literature and other teaching material Scientific publications covering the techniques covered will be distributed at the start of the course.
Number of students 9 - 18
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information KI Solna, KI Huddinge, KS. 9:00 - 16:00, Monday - Friday.
This course has previously been given with number 1559.
Additional course leader
Latest course evaluation Course evaluation report
Course responsible Alexander Chibalin
Institutionen för molekylär medicin och kirurgi
0852487512
Alexander.Chibalin@ki.se
Contact person -