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Title Genetic Engineering Techniques
Course number 3172
Programme 1-Ingår i flera program
Language English
Credits 3.0
Date 2019-08-26 -- 2019-09-06
Responsible KI department Institutionen för mikrobiologi, tumör- och cellbiologi
Specific entry requirements
Purpose of the course The functional analysis of microbial genomes from the characterization of virulence components to characterization of secondary metabolite modules relies heavily on genetic manipulation techniques. The purpose of the course is to present and implement state-of-the-art genetic techniques for the manipulation of microbial genomes in research lectures and practical sessions using model organisms and multicellular microbial behavior (biofilm formation) as a model system. This course will also provide the participants with the theoretical knowledge to develop genetic manipulation techniques in novel microbial organisms and in the context of novel model systems. The participants will be made aware of the ethical, legislative and safety aspects of genetic manipulation techniques.
Intended learning outcomes At the end of the course the student should be able to:

Knowledge/abilities:
- independently carry out the applied state-of-the-art genome manipulation techniques in the microbial model system(s)
- have knowledge about the various alternative random and specific genome manipulation approaches
- follow and critically judge the literature on the development of specific genome manipulation tools
- theoretically and practically develop genome manipulation approaches for novel model organisms
- theoretically and practically develop novel genome manipulation tools
- have knowledge about the biological background and molecular mechanisms of genome manipulation tools
- have knowledge about genetechnically modified organisms (GMO) legislation

Approaches:
- deliberately choose the appropriate tool for the desired genome manipulation
- deliberately develop suitable genome manipulation tools
- deliberately practically implement GMO legislation for a safe working environment
Contents of the course Lectures on genetic tools, their original underlying biological impact and molecular mechanisms of action. As such, the lectures will cover, for example, strategies to develop manipulation strategies for organisms, cloning strategies, CRISPR/Cas for microbial and eukaryotic genome manipulation, recombinases and recombination, transposases, DNA replication, DNA repair mechanisms and restriction-modification systems. Hands-on work including, for example, in vivo cloning, single nucleotide exchange strategies to random transposon mutagenesis will be covered.
Teaching and learning activities Lectures, laboratory work, documentation, seminars, IT-applications and home work.
Compulsory elements Lectures, laboratory work and seminars are compulsory. Absence (less than 10% of course time) can be compensated for by the performance of alternative elements according to agreement.
Examination Examination will be in the form of written documentation of experimental results and discussion, oral presentation of results and a short written questionnaire.
Literature and other teaching material Thomason LC, Sawitzke JA, Li X, Costantino N, Court DL. Recombineering: genetic engineering in bacteria using homologous recombination. Curr Protoc Mol Biol. 2014, 106:1.16.1-39. van Kessel JC, Hatfull GF. Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets. Mol Microbiol. 2008, 67(5):1094-107. Tellier M, Bouuaert CC, Chalmers R. Mariner and the ITm Superfamily of Transposons. Microbiol Spectr. 2015, 3(2):MDNA3-0033-2014.. doi: 10.1128/microbiolspec.MDNA3-0033-2014. Thomason LC, Costantino N, Court DL. Examining a DNA Replication Requirement for Bacteriophage ¿ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli. MBio. 2016, 7(5). pii: e01443-16. A laboratory manual and additional literature will be distributed at the beginning of the course.
Number of students 10 - 30
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information Course hours: 9:00-16:30, Monday to Friday.

Course address: Scheele laboratory, Scheeles väg 2, Campus Solna

The course is given jointly by the doctoral programmes Cell Biology and Genetics (CBG) and Biology of infections and global health (BIGH), https://ki.se/en/staff/doctoral-programmes.
Additional course leader Mark Gomelsky, Jim Sawitzke.
Earlier evaluation of the course Not available
Course responsible Ute Römling
Institutionen för mikrobiologi, tumör- och cellbiologi
0852487319
Ute.Romling@ki.se
Contact person Shady Kamal
Institutionen för mikrobiologi, tumör- och cellbiologi

shady.kamal@ki.se