Course catalogue doctoral education - VT18

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Title In situ hybridization: theory and practice
Course number 2872
Program Kardiovaskulär forskning
Language English
Credits 1.5
Date 2017-11-27 -- 2017-12-01
Responsible KI department Institutionen för medicin, Solna
Specific entry requirements
Purpose of the course In situ hybridization is a technique widely-used in many research fields, but it is challenging to set up. The purpose of this course is to enable the students to master this technique and be able to apply this tool in their own research projects.
Learning outcomes At the end of the course the student should be able:
1. to describe the theoretical background behind in situ hybridization techniques. Discuss pros and cons in relation to the biological problem studied.
2. to design and setup in situ experiment based on her/his own research project, including optimization of conditions of the key steps during the experiment.
3. to perform and document experimental work in accordance with Good Laboratory Practice (GLP) guidelines.
4. to communicate the results in writing and orally.
Contents of the course The course will cover the basics about theory and practice of in situ hybridization techniques. The focus will be on demonstrating and explaining current in situ hybridization techniques to detect RNAs (microRNA, long-non-coding RNA and mRNA) and DNA molecules in tissues / cells. The participant will get hand-on practice on in situ hybridization technique.
Teaching and learning activities The course will start with introductory lectures, in which the invited technique experts will talk about the theoretical background and the cutting-edge progress about in situ hybridization techniques. The course participants will have a chance to perform an in situ hybridization experiment under supervision to detect RNAs in tissues. The course will end with an interactive exercise, discussing the theory and the lab data in addition to the application of in situ hybridization technique in the participants' own research projects.
Compulsory elements All parts are mandatory. Absence from a lecture or discussion may be compensated by a written assignment, on agreement with the course leader.
Examination Interactive exercise discussing how to apply in situ hybridization techniques in the participants' own research projects taking the theoretical aspects regarding in situ hybridization into account. Every student will be individually assessed.
Literature and other teaching material Relevant publications covering the topic will be handed out by lecturers.
Number of students 8 - 12
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information This course will be co-funded by the Ming Wai Lau Center of Reperative Medicine (MWLC), ki.se/mwlc.
Additional course leader
Earlier evaluation of the course Evaluation report
Course responsible Ning Xu
Institutionen för medicin, Solna

ning.xu@ki.se
Contact person -