Kurskatalog forskarutbildning - HT18

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Titel In situ hybridization: theory and practice
Kursnummer 2872
Program Kardiovaskulär forskning
Språk Engelska
Antal högskolepoäng 1.5
Datum 2017-11-27 -- 2017-12-01
Kursansvarig institution Institutionen för medicin, Solna
Särskild behörighet
Kursens syfte In situ hybridization is a technique widely-used in many research fields, but it is challenging to set up. The purpose of this course is to enable the students to master this technique and be able to apply this tool in their own research projects.
Kursens lärandemål At the end of the course the student should be able:
1. to describe the theoretical background behind in situ hybridization techniques. Discuss pros and cons in relation to the biological problem studied.
2. to design and setup in situ experiment based on her/his own research project, including optimization of conditions of the key steps during the experiment.
3. to perform and document experimental work in accordance with Good Laboratory Practice (GLP) guidelines.
4. to communicate the results in writing and orally.
Kursens innehåll The course will cover the basics about theory and practice of in situ hybridization techniques. The focus will be on demonstrating and explaining current in situ hybridization techniques to detect RNAs (microRNA, long-non-coding RNA and mRNA) and DNA molecules in tissues / cells. The participant will get hand-on practice on in situ hybridization technique.
Arbetsformer The course will start with introductory lectures, in which the invited technique experts will talk about the theoretical background and the cutting-edge progress about in situ hybridization techniques. The course participants will have a chance to perform an in situ hybridization experiment under supervision to detect RNAs in tissues. The course will end with an interactive exercise, discussing the theory and the lab data in addition to the application of in situ hybridization technique in the participants' own research projects.
Obligatoriska moment All parts are mandatory. Absence from a lecture or discussion may be compensated by a written assignment, on agreement with the course leader.
Examination Interactive exercise discussing how to apply in situ hybridization techniques in the participants' own research projects taking the theoretical aspects regarding in situ hybridization into account. Every student will be individually assessed.
Kurslitteratur och övriga läromedel Relevant publications covering the topic will be handed out by lecturers.
Antal studenter 8 - 12
Urval av studenter Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
Övrig information This course will be co-funded by the Ming Wai Lau Center of Reperative Medicine (MWLC), ki.se/mwlc.
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Kursansvarig Ning Xu
Institutionen för medicin, Solna

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