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| Titel | Human Cell Culture. Methods and Applications |
|---|---|
| Kursnummer | 1202 |
| Program | X- Används inte längre - Miljöfaktorer och hälsa |
| Språk | Engelska |
| Antal högskolepoäng | 1.5 |
| Datum | 2017-09-04 -- 2017-09-08 | Kursansvarig institution | Institutet för miljömedicin |
| Särskild behörighet | |
| Kursens syfte | The purpose of the course is to enable doctoral students to acquire state-of-the art knowledge and good understanding of human cell culture. |
| Kursens lärandemål | After the course the students should be able to: Describe the theory and give examples of practical applications of human cell culture Explain basic and state-of-the-art methods applied to cell cultures Discuss possibilities and challenges in cell culture work |
| Kursens innehåll | Cell culture reflecting stem, transit amplifying, differentiated and terminally differentiated tissue states. Monolayer and organotypic culture involving one or more cell types. Applicability of cell cultures as alternatives to laboratory animal experiments. Mechanisms regulating cell growth and viability, differentiation and apoptosis. Assessment of cell transformation to immortal and malignant phenotypes. Isolation of specific cells, e.g., epithelial cells, characterisation of cultured cells. Handling and sterile techniques, choice of materials and media for cell culture, e.g., serum-dependent vs. serum-free culture conditions. Cell cloning and gene transfer. Practical handling of cultures: thawing/freezing, passage, expansion and long-term storage. Handling of normal and tumor tissue for optimizing obtainment of cultures. High-throughput screening technologies. Tissue engineering practices. Transcriptomics, proteomics and informatics methods for biomedical research with cell lines. Discussion of participants' own culture experience and problems. |
| Arbetsformer | Interactive lectures, laboratory work, computer exercises and group discussions on pitfalls and possibilities with cell cultures. |
| Obligatoriska moment | Participation in interactive lectures, group discussions, laboratory work and oral examination is compulsory. Absence from compulsory elements can compensated by participation at the next course occasion. |
| Examination | Examination is in the form of a written assignment and oral presentation. |
| Kurslitteratur och övriga läromedel | Handouts are provided for all elements of the course. |
| Antal studenter | 8 - 15 |
| Urval av studenter | Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date) |
| Övrig information | The course is held at KI Campus Solna. |
| Ytterligare kursledare | |
| Senaste kursvärdering | Kursvärderingsrapport |
| Kursansvarig |
Roland Grafstrom Institutet för miljömedicin Roland.Grafstrom@ki.se |
| Kontaktpersoner |
Johanna Bergman Institutet för miljömedicin johanna.bergman@ki.se Nobels väg 13 17177 Stockholm |
