Course catalogue doctoral education - VT18

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Title Human Cell Culture. Methods and Applications
Course number 1202
Program Miljöfaktorer och hälsa
Language English
Credits 1.5
Date 2017-09-04 -- 2017-09-08
Responsible KI department Institutet för miljömedicin
Specific entry requirements
Purpose of the course The purpose of the course is to enable doctoral students to acquire state-of-the art knowledge and good understanding of human cell culture.
Learning outcomes After the course the students should be able to:
Describe the theory and give examples of practical applications of human cell culture
Explain basic and state-of-the-art methods applied to cell cultures
Discuss possibilities and challenges in cell culture work
Contents of the course Cell culture reflecting stem, transit amplifying, differentiated and terminally differentiated tissue states. Monolayer and organotypic culture involving one or more cell types. Applicability of cell cultures as alternatives to laboratory animal experiments. Mechanisms regulating cell growth and viability, differentiation and apoptosis. Assessment of cell transformation to immortal and malignant phenotypes. Isolation of specific cells, e.g., epithelial cells, characterisation of cultured cells. Handling and sterile techniques, choice of materials and media for cell culture, e.g., serum-dependent vs. serum-free culture conditions. Cell cloning and gene transfer. Practical handling of cultures: thawing/freezing, passage, expansion and long-term storage. Handling of normal and tumor tissue for optimizing obtainment of cultures. High-throughput screening technologies. Tissue engineering practices. Transcriptomics, proteomics and informatics methods for biomedical research with cell lines. Discussion of participants' own culture experience and problems.
Teaching and learning activities Interactive lectures, laboratory work, computer exercises and group discussions on pitfalls and possibilities with cell cultures.
Compulsory elements Participation in interactive lectures, group discussions, laboratory work and oral examination is compulsory. Absence from compulsory elements can compensated by participation at the next course occasion.
Examination Examination is in the form of a written assignment and oral presentation.
Literature and other teaching material Handouts are provided for all elements of the course.
Number of students 8 - 15
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information The course is held at KI Campus Solna.
Additional course leader
Earlier evaluation of the course Evaluation report
Course responsible Roland Grafstrom
Institutet för miljömedicin

Roland.Grafstrom@ki.se
Contact person Johanna Bergman
Institutet för miljömedicin

johanna.bergman@ki.se

Nobels väg 13

17177
Stockholm