Kurskatalog forskarutbildning - VT24

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Titel Surgical Techniques in Rat and Mouse
Kursnummer 2647
Program 0-Inte del av forskarutbildningsprogram
Språk Engelska
Antal högskolepoäng 2.0
Noteringar Kursen är en försöksdjurskurs.
Datum 2017-10-09 -- 2017-10-13
Kursansvarig institution Komparativ medicin
Särskild behörighet Students need to have completed a laboratory animal science course on how to carry out scientific procedures on the appropriate animal species i.e. EU Function A or equivalent course.
Kursens syfte Training in surgery is both essential, and a legal requirement, for all those who need to undertake surgical procedures on mice and rats used in research. Applying appropriate techniques to in vivo studies enhances outcomes from research studies, reduces data variability, and is perceived as ethically acceptable. The course includes training sessions covering both advanced and special surgical techniques.
Kursens lärandemål The course is designed to meet the learning outcomes specified by the training recommendations supplied as an annex to EU Directive 2010/63/EU. Swedish legislation was amended to meet the requirements of this Directive in 2013. After completion of this course, the students should be able to meet the defined learning outcomes as set out in the EU modules 20, 21 and especially in module 22. Each module requires both theoretical knowledge, and acquisition and demonstration of practical skills. The list of suggested learning outcomes by the endorsed guidelines is comprehensive, but in summary, participants will acquire the knowledge and skills to anaesthetize animals safely and humanely, assess and alleviate post-surgical pain, and be able to conduct routine, advanced and special surgical procedures competently. In brief, after the course the participants are expected to be able to: 1) Handle pre-, peri- and post-operative care of rats and mice, including asepsis and analgesia; 2) Recognize microsurgery instruments and their use; 3) Set and use a microsurgery microscope; 4) Use appropriate suture materials; 5) catheterize blood vessels and to collect blood.
Kursens innehåll Analgesia and perioperative care, aseptic surgical techniques, basic and advanced surgical techniques including instrumentation, suturing and tissue handling, vein and artery cannulation, explorative laparotomy and blood sampling techniques. Rat will be our main animal model during the course (as techniques are easier to learn in a larger model), but mouse will also be available.
Arbetsformer A minor part of the learning activities are lectures (10%). The laboratory activities account for 70% of the course. The remaining part of learning activities are demonstrations and project work (tutoring available).
Obligatoriska moment All sessions and active student participation are compulsory if the student is to be provided with certification of the successful completion of the course. Missed parts of the course as a consequence of a well-justified absence will need to be compensated after agreement with the course director e.g. with written assignment or in future course occasions.
Examination Practical skills are assessed during the laboratory session using DOPS (direct observation of practical skills), and a short answer/multiple choice question final written examination is held following conclusion of the course. A pass/fail criteria will be used as a global rate for this course, a level of supervision for the prospective work on animals will be assigned, as suggested in the EU guidelines endorsed by the Swedish competent authority for the protection of animals used in science.
Kurslitteratur och övriga läromedel Detailed notes (compiled by the lecturer) are provided, that support all of the specified learning outcomes.

1. Remie, R. (1990). Manual Microsurgery on the Laboratory Rat. Elsevier Science. ISBN 0444811397
2. Flecknell, P.A. (2015) Laboratory Animal Anaesthesia, 4th Edition, Elsevier, New York.
3. Krinke, G.J. (2000). The Laboratory Rat (Handbook of experimental animals). Academic Press. ISBN 012426400X
4. Hedrich, H. (2004), The Laboratory Mouse (Handbook of experimental animals). Academic Press. ISBN 0123364256
5. Waynforth, H.B., Flecknell, P.A. (1992). Experimental and surgical techniques in the rat. Academic Press. ISBN 0127388516
6. National Research Council of the National Academies (2009). Recognition and Alleviation of Pain in Laboratory Animals, National Academies Press, Washington D.C.
7. Aseptic Technique in Rodent Surgery: http://www.procedureswithcare.org.uk/aseptic-technique-in-rodent-surgery/
Antal studenter 8 - 16
Urval av studenter Selection will be based on the relevance of the course syllabus for the applicant's doctoral project (need to use surgical or anaesthetic techniques in rodent models), which will be according to written motivation. If necessary, additional selection crterium will be used based on the date for registration as a doctoral student (priority given to earlier registration date).
Övrig information The course is held from Monday to Friday between 9 am and 5 pm. Location: Learning Lab, von Eulers väg 4A, 2nd floor. Key topics of this course include surgical and anaesthetic procedures on rats and mice, with focus on microsurgery and advanced surgical skills in rodent models. The main instructor of this course is internationally-recognized Professor René Remie, PhD, author and editor of the Manual of Microsurgery on the Laboratory Rat.
Ytterligare kursledare Rafael Frías | DVM, MSc (LAS), PhD Director, LAS Education & Training Unit Comparative Medicine | Karolinska Institutet 171 77 Stockholm, Sweden | Von Eulers väg 4A, 4th floor T: +46 8524-86660 | M: +46 737 874-103 rafael.frias@ki.se | internwebben.ki.se/las-courses
Senaste kursvärdering Kursvärderingsrapport
Kursansvarig Rafael Frias
Komparativ medicin
085246660
rafael.frias@ki.se
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