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Title Digital photomicroscopy
Course number 2904
Programme 0-Inte del av forskarutbildningsprogram
Language English
Credits 1.5
Date 2017-10-09 -- 2017-10-13
Responsible KI department Institutionen för laboratoriemedicin
Specific entry requirements
Purpose of the course The purpose of this course is to teach the students how to handle a microscope and to document the results of research in digital form. The ethics regarding adjusting/clarifying or manipulating an image will also be discussed.
Intended learning outcomes After completing the course, the participants will have a working knowledge of the fundamentals of optical microscopy and be able to use appropriate presentation methods for conveying their research results in a clear and informative way. The participants will be able to differ between clarification and manipulation, and will be able to clarify their images without altering them. Image processing software will be used, inlcuding Adobe Photoshop® as well as other digital microscopy software for image aquisition. The participants will have a sound foundation for their work in adjusting their images for publication in a responsible and honest manner.
Contents of the course The course will include theory and practice investigating conventional-light microscopy, dark field, ultraviolet fluorescence, phase contrast and interference (DIC).

Additionally, the course will dedicate one day to confocal microscopy and the analysis and measurement of data through theoretical and practical sessions. Following the mastery of microscopy, the course explores fundamentals of digital photography. The participants wil be exposed to digital cameras and their proper operation. There is an overview of video and still digital cameras relative to application needs. Relevant issues such as capture, processing transmission and storage concerns are explored.

Participants will work individually with popular editing software such as Adobe Photoshop® as well as other imaging software. Excercises have been created to develop imaging processing imperatives such as file resolution and type, adjusting of tones and contrast, and composition. The participants will learn the difference between clarifying an image and altering it.
Teaching and learning activities The course will consist of lectures and practical microscopy laborations.
Compulsory elements Practical microscopy sessions are compulsory, as is an overall attendance of 100%. Absence can be compensated for by completing extra work as agreed with the course organizer.
Examination Each participant's knowledge will be assessed through an oral examination during a practical microscopy laboration.
Literature and other teaching material Compendia on microscopy and on editing software.
Number of students 10 - 18
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information The course will be held 08:30-17:00 Monday through Friday. Information regarding the venue will be sent to course participants well ahead of time.
Additional course leader
Latest course evaluation Course evaluation report
Course responsible Moustapha Hassan
Institutionen för laboratoriemedicin
08-585 838 62
Moustapha.Hassan@ki.se
Contact person Jennifer Usterud
Institutionen för laboratoriemedicin
08-585 832 38
jennifer.usterud@ki.se