Course catalogue doctoral education - VT24

    Startpage
  • Ansökan kan ske mellan 2023-10-16 och 2023-11-15
Application closed
Print
Title Human embryonic stem cells
Course number 2212
Programme Utveckling och regeneration (DevReg)
Language English
Credits 1.5
Date 2017-09-04 -- 2017-09-08
Responsible KI department Institutionen för biovetenskaper och näringslära
Specific entry requirements
Intended learning outcomes The objectives of this course are that at the conclusion of this course students should have a good understanding of:
- Folliculogenesis and Fertilization
- Pre implantation Embryology
- Criteria of selected or scoring the blastocysts for isolation of ICM
- Derivation methods of hESCs
- Culture condition of hESCs
- Main components of the culture system (culture media, tissue culture plastics, gases, incubators, workstations, laboratory environment, input materials)
- Physical-chemical properties of culture system (osmolarity and pH of culture media, temperature, light levels) and how they can be influenced
- Nutritional requirements of the blastocyst and hESCs
- Functional characteristics of different tissue culture incubators
- The influence of the laboratory and clinic environment on hESCs
- Developmental milestones
- Students should know the prospective possibilities of having a good culture system
- Students should be aware of the general aspects and implication of the stem cells research and the potentiality that these represent for clinical application
- Characterization of the embryonic stem cells and the importance of the pluripotency of these cells
- The different differentiations assay on stem cells and what is ongoing in this field
- The immunorejection problem and the production of isogonics embryonic stem cells by somatic cell nuclear transfer or therapeutic clone (SCNT)
- The pluripotence induction of somatic cell by transduction, (the iPS cells)
- Finally the students will improve their capacity to produce coherent, logical and concise explanations of data and concepts - both written and oral, through consideration of the course material.
- Students will also develop their ability to criticize scientific literature related with hESC technology and reproduction physiology in a constructive and informed fashion
- Be aware of potential development of hESC technology in the future.
Contents of the course Divide in lectures and laboratory work

1. Embryology ¿theory¿ (lectures)
2.Culture system (lectures)
3.Human Embryonic Stem cells culture (lectures)
4. Characterization (lectures)
5. Laboratory demonstrations
6. Genetics (lectures)
7. Induction of different cell types (lectures)
8. Reprogramming (lectures)
9. Stem cells therapy and challenge (lecture)
10. Future in human embryonic stem cells (lecture)

Course test, evaluation, discussions, examination and closing of course:
Teaching and learning activities The course (human embryonic stem cells) runs for one week every year, with lectures and laboratory practical demonstrations.
Compulsory elements The laboratory parts are obligatory. If absent at laboratory activity; student should present a literature work related with the subject of the missing activity
Examination Writing individual- examen
Literature and other teaching material Lecturers with expert knowledge in the field of human embryonic stem cell technology will give presentations on the selected subjects.
Stem cell Research: Medical application & Ethical controversy, Joseph Panno, The New Biology, 2005
Human Embryology, William J. Larsen, Third Edition, Churchill Livinstone, 2001
Manipulating the mouse embryos ""Laboratory manual"" third edition Andras Nagy, Marina Gertsenstein, Kristina Vintersten, Richard Behringer, 2003
IVF LAB ""Laboratory aspects of in-vitro fertilization"" Editor: M. Bras, J.W. Lens, M. H. Piederiet, P.M. Rijinders, M. Verveld, G.H. Zeilmaker, 1996
Number of students 8 - 15
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information The course will be held at Karolinska Institutet, Department of Biosciences and Nutrition, NOVUM, Huddinge.
Additional course leader
Latest course evaluation Course evaluation report
Course responsible Jose Inzunza
Institutionen för biovetenskaper och näringslära
08-585 850 93
Jose.Inzunza@ki.se

Hälsovägen 7, Novum

141 86
Stockholm
Contact person Jose Inzunza
Institutionen för biovetenskaper och näringslära
08-585 850 93
Jose.Inzunza@ki.se

Hälsovägen 7, Novum

141 86
Stockholm