Course catalogue doctoral education - VT18

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Title Fluorescence microscopy: High content image acquisition and analysis
Course number 2973
Program Cellbiologi och genetik
Language English
Credits 3.0
Date 2017-10-02 -- 2017-10-13
Responsible KI department Institutionen för cell- och molekylärbiologi
Specific entry requirements
Purpose of the course Fluorescence microscopy is for most researchers an essential tool. Lately, this technique developed more and more from 'simply' acquiring good looking images towards complex, high content imaging techniques.
High content imaging is defined by the large number of image data generated either from live cell imaging, or microscopy on fixed samples. Examples are z-stack imaging, cell migration, protein dynamics, multi-position imaging, tiling & stitching, whole mount imaging, and automated image acquisitions in screening assays. The purpose of this course is to make the participants familiar with all of these high content techniques; from acquisition to analysis and presentation.
Learning outcomes After passing the complete course, the participants will be familiar with diverse high content fluorescence microscopy applications. They will be competent in designing and performing experiments involving high content fluorescence microscopy. The participants will be able to analyse their data using an image analysis software, and how to present the data in a scientific format.
Contents of the course Basic principles of fluorescence microscopy. Confocal Laser Scanning Microscopy: z-stack, tiling & stitching. Live Cell Imaging: long term imaging, protein dynamics (FLIP/FRAP). Automated Image Acquisition Microscope: multi-well imaging, screening. Hands-on sessions with diverse high content imaging experiments. Image analysis: ImageJ/CellProfiler. Lectures and workshops. Poster presentation
Teaching and learning activities The pedagogic learning activities in the course consist of lectures, research seminars, hands-on experience at the microsopes/imaging, group discussions, experimental design, data processing and poster presentation.
Compulsory elements All activities (lectures, research seminars, microscope sessions, group discussions, data processing and poster presentations) are compulsory. If students are unable to attend they have to discuss with course organizer how this can be compensated. Compensation of microscopy sessions is not possible.
Examination There will be 2 examinations. In the end of the first week, there will be an assessment of written versions of experimental designs involving high content imaging experiments and image analysis. In the second week, the participants will present a scientific poster showing the results of their high content microscopy experiments performed during that week. The poster presentations will be attended by course participants, lecturers, course assistants, and others that are interested.
Literature and other teaching material
Number of students 12 - 18
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information The course content will cover: Basic principles of fluorescence microscopy; Confocal Laser Scanning Microscopy: z-stack, tiling & stitching; Live Cell Imaging: long term imaging, protein dynamics (FLIP/FRAP); Automated Image Acquisition Microscope: multi-well imaging, screening; Hands-on sessions with diverse high content imaging experiments; Image analysis: ImageJ/CellProfiler lectures and workshops; Poster presentation. The course takes place at CMB, seminar room A216, Berzelius v 35 with entrance from the court yard of CMB.
Additional course leader The course director is Florian Salomons, PhD Department of Cell & Molecular Biology Florian.Salomons@ki.se. The course co-director is Victoria Menendez Benito, PhD Department of Biosciences and Nutrition Victoria.Menendez.Benito@ki.se.
Earlier evaluation of the course Not available
Course responsible Florian Salomons
Institutionen för cell- och molekylärbiologi
0852487395
Florian.Salomons@ki.se
Contact person Matti Nikkola
Institutionen för cell- och molekylärbiologi

Matti.Nikkola@ki.se