Course catalogue doctoral education - VT18

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Title Calcium signaling
Course number 2733
Program Utveckling och regeneration (DevReg)
Language English
Credits 1.5
Date 2017-09-04 -- 2017-09-08
Responsible KI department Institutionen för klinisk forskning och utbildning, Södersjukhuset
Specific entry requirements
Purpose of the course The aim of this course is to enable the students to get an insight in the fundamental mechanisms of the regulation of cellular Ca2+ homeostasis, the generation and the decoding of Ca2+ signaling, the principles of the methods for measuring Ca2+ concentrations in different cellular compartments, and the general roles of Ca2+ signals in mediating cellular functions.
Learning outcomes After the course the students should be able to:
1. Critically analyze and interpret how the different constituents of the Ca2+ signaling tool-kit participate in the generation and decoding of Ca2+ signals, and in maintaining Ca2+ homeostasis.
2. Choose appropriate methods for studying different aspects of Ca2+ signaling
3. Critically analyze the existing literature on Ca2+ signaling, generate new ideas and put forward new hypotheses.
4. Design new studies on Ca2+ signaling in the context that is relevant to the research areas of the students themselves
Contents of the course 1. Phospholipase C and inositol 1,4,5 trisphosphate-mediated signaling. 2. Identity and roles of the molecular players involved in Ca2+ and phospholipid-mediated signaling. 3. Preparation of Ca2+ buffers. 4. Principles of methods used in the study of calcium and phospholipid signaling including fluorescent techniques, electrphysiology and imaging techniques. 5. Regulation of ion channels involved in Ca2+ signaling including voltage sensitive channels, Transient Receptor Potential channels, store- operated channels and intracellular C a2+ channels. 6. Roles of Ca2+ and phospholipid mediated signaling in cellular processes including in secretion and apoptosis. 7. Mechanism of generation and decoding of Ca2+ signals. 8. Spatial and temporal aspects of Ca2+ signaling. 9. How to pick research problems in the areas of Ca2+ and phospholipid signaling and how to approach the problems.
Teaching and learning activities This course will follow the principles of active learning including the seven steps problem-based-learning (PBL), and flipped classrooms. Emphasis will be on self-directed learning through problem-solving in small groups rather than on cathedral lectures. Generous small-group interactive "lecture sessions" by resource personnel with ample time for questions and answers will be provided. Participants will work on selected problems designed to be starting points, in groups of about ten participants, under supervision of trained facilitators who will be available during all of the sessions. Participants will be provided with an outline of the objectives, areas expected to cover, and reprints of selected learning materials.
Compulsory elements Attendence in all the sessions is obligatory. In case of absence, the participant will have to submit written reports specified by the course-supervisor, to compensate for the absence.
Examination Each student must submit a research proposal in the area of Ca2+ signalling where they will critically analyze the existing literature, identify the gaps in the existing knowledge, put forward a new hypothesis, and choose appropriate methods to test the hypothesis. They will use conventional headings like: specific aims, background, methods, significance, and references. The proposal should be at least one A4 page long, but no longer than three pages.
Literature and other teaching material Recommended literature: 1. Calcium signaling. Advances in Experimental Medicine and Biology, Vol 740, 2012. Springer. Md. Shahidul Islam (Editor) 2. Calcium Techniques: A Laboratory Manual by Jan B. Parys (Editor), Martin Bootman (Editor), David I. Yule (Editor), Geert Bultynck (Editor). 2013. Cold Spring Harbor Laboratory Press 3. In addition, reprints of selected learning materials will be distributed.
Number of students 10 - 20
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information The course will be held at the Department of Clinical Science and Education, Södersjukhuset (Metro Skanstull, Buss number 3 to the entrance of the Södersjukhuset). The course will start every day at 09:00 and end by latest 16:00. Lunch break 12:00-13:00.
Additional course leader Joseph Bruton, Department of Physiology and Pharmacology.
Earlier evaluation of the course Evaluation report
Course responsible Shahidul Islam
Institutionen för klinisk forskning och utbildning, Södersjukhuset
086163950
Shahidul.Islam@ki.se
Contact person -