Course catalogue doctoral education - HT17

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Title Applications of CRISPR/Cas9 technology: genome editing and beyond
Course number 2970
Program Tumörbiologi och onkologi (FoTO)
Language English
Credits 1.5
Date 2017-10-09 -- 2017-10-13
Responsible KI department Institutionen för mikrobiologi, tumör- och cellbiologi
Specific entry requirements
Purpose of the course CRISPR/Cas9 technology provides a precise and easy way for genome manipulation in cells or in organisms, and therefore is an extremely powerful tool for research. This course aims to help students to understand the CRISPR/Cas9 system and its broad range of applications in research and clinics, and how to use this tool for their own research.
Learning outcomes After the completed course, the students should have knowledge and understanding of (1) the mechanism of CRISPR/Cas9 molecular machinery; (2) how to use CRISPR/Cas9 technology in eukaryotic cells to edit genomes and what are its limitations; (3) what other applications the system has in research as well as in clinics; (4) ethical issues and concerns related to the application of this technology. In term of technical skills, at the end of the course the students should be able to design guide RNA to target specific loci of interest for their own research
Contents of the course The course will include lectures by invited experts about the CRISPR/Cas9 technology, how to use it for genome editing in cells or organisms, as well as examples of exploitation for different applications in research (such as cell imaging, whole genome screening approaches and transactivation/repression of genes) and in clinics. A computer session will be done for the student to design gRNA for their own CRISPR/Cas9 based approach. In seminars, the students will present and discuss recent literature about CRISPR/Cas9 technology.
Teaching and learning activities The learning activities of the course will consist of lectures from invited experts in the morning and seminars in the afternoon. In addition, one practical computer session will give to the students the opportunity to design CRISPR/Cas9-based strategies for their own research. A problem-solving session will also be part of the course. In group of three, the students will have to design a strategy to solve a given problem and present it to others at the end of the course.
Compulsory elements Presence at lectures, seminars, and final presentation and discussion is mandatory. To compensate for absence a written essay on the missed topic shall be performed.
Examination Examination will consist of an oral presentation in which students, in group of three, will present their CRISPR-Cas9 based strategy to answer a given problem.
Literature and other teaching material Literature will consist of recent original and review articles and will be sent to students as PDF files
Number of students 8 - 20
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information The course will take place at Dept. of Microbiology, Tumor and Cell Biology (MTC) (Nobels väg 16, Solna Campus). More information about the exact schedule will be provided to course participants before the beginning of the course.
Course directors Director: Sylvain Peuget (sylvain.peuget@ki.se) Co-director: Galina Selivanova (galina.selivanova@ki.se)
Earlier evaluation of the course Not available
Course responsible Sylvain Peuget
Institutionen för mikrobiologi, tumör- och cellbiologi

sylvain.peuget@ki.se
Contact person -