Course catalogue doctoral education - HT17

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Title Methods in molecular biology and their applications in medical research
Course number 2418
Program Metabolism och endokrinologi
Language English
Credits 3.0
Date 2017-09-04 -- 2017-09-15
Responsible KI department Institutionen för laboratoriemedicin
Specific entry requirements
Purpose of the course The aim of this course is to confer knowledge of how basic molecular biology and protein work can be applied in clinically oriented research. The course is designed for doctoral students with limited experience of molecular biology and combines a lecture program with laboratory work. The course combines theoretical knowledge with hands-on practice.
Learning outcomes After the course, the students will understand the principles, from both a theoretical and practical viewpoint, of a number of basic techniques used in DNA/protein research and their application in medical research. The students should also be able to design and perform investigations with some of the discussed techniques.
Contents of the course The lectures will include theoretical and practical aspects on basic concepts concerning techniques in molecular biology and proteomics and their application in medical research. Specified subjects will include cloning, RNA-analysis, promoter studies, DNA-sequencing, expression, bioinformatics, antibody-based protein detection and proteomics. The overall objective is to convey knowledge of possibilities and limitations with different techniques and introduce valuable methodology for the students' PhD-projects.
Teaching and learning activities The course combines a lecture program in the morning with laboratory work in the afternoon, where course participants will be subdivided into smaller groups.
Compulsory elements Laboratory work is compulsory. To compensate for absence, self-studies and a written essay on the topic(s) that they have missed must be performed.
Examination The students will be asked to solve a scientific problem using methods they learned during the course. The last day of the course, the participants will give a presentation. They should be able to defend the laboratory design, discuss methodologies applied and anticipated results. The students will work in pairs. The task will be given to the participants at the end of the first week. Every doctoral student will be individually assessed.
Literature and other teaching material Reprints and articles will be provided by the course administrators and the responsible lecturers.
Number of students 8 - 16
Selection of students Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
More information
Course directors
Earlier evaluation of the course Evaluation report
Course responsible Tomas Jakobsson
Institutionen för laboratoriemedicin

Tomas.Jakobsson@ki.se
Contact person Veronika Tillander
Institutionen för laboratoriemedicin

Veronika.Tillander@ki.se