Course catalogue doctoral education - HT17

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Title Flow cytometry: from theory to application
Course number 1496
Program Kardiovaskulär forskning
Language English
Credits 1.5
Date 2017-09-25 -- 2017-09-29
Responsible KI department Institutionen för medicin, Solna
Specific entry requirements
Purpose of the course The course aims to provide doctoral students with: 1) a systematic introduction of flow cytometry, 2) an overview of flow cytometric applications in biomedical research, and 3) a hands-on opportunity of flow cytometry lab training designed for various levels of flow cytometer users.
Learning outcomes Through this one-week course, the participants will acquire the knowledge of flow cytometry principles. They will know flow cytometric sample preparation and analysis, protocol set-up, major flow cytometric applications, as well as data interpretation and presentation.
Contents of the course The course covers basic knowledge and main applications of flow cytometry, and contains both lectures and laboratory/group work. Experts in different fields of flow cytometric application are invited to give the lectures. The participants will be able to learn: a) Principles of flow cytometry: working mechanisms of flow cytometer; molecular and cellular probes for flow cytometry; quality control; and safty procedures and intrument maintainance. b) Basic skills: flow cytometric protocol design; sample handling and storage; data processing and analysis, and data presentation. c) Major applications of flow cytometry in biomedical research: leukocyte phenotyping, endothelial cell analysis, stem cell analysis, apotosis, platelet measurement, cell function analysis (including membrane potential, oxidative metabolism, intracellular calcium mobilization, pH, and intracellular organelles), as well as nucleic acid analysis.
Teaching and learning activities The course includes 28 hr lectures and 12 hr hands-on lab work.
Compulsory elements The participants must attend hands-on lab sections during two afternoons. The students who have missed these sections can book extra lab section time within 4 weeks to compensate the absence.
Examination The exam will consist two parts: a 30-min written test on flow cytometric principles; and a 60 min hands-on lab work examining flow cytometric lab skills
Literature and other teaching material The course will provide organized handouts from all lectures.
Number of students 20 - 32
Selection of students Selection will be based on: 1) The relevance of flow cytometry for the applicant's project (according to written motivation) 2) Urgency of flow cytometry training for the applicant's project (according to written motivation) 3) Previous application (priority given to those who have applied previously)
More information Course held during week 39 (Sept 25 -29, 2017; 9:00-16:30). Lecture hall booked: Block salen, T4:00 at Karolinska University Hospital-Solna. Lectures will be given by 14-15 lecturers, usually half of them from institutions outside KI, with one or two from abroad. All the lecturers are well-established experts in their lecture subjects of flow cytometric applications. The course has been given 1-2 times/year at KI for 16 years. It has been highly appreciated by the participants through the years, with a median general satisfaction score never below 8 (out of 9; referred to paper-based course evaluation between 2001-2013), and highly remarked with the web-based course evaluations (2014-2016).
Course directors Course organizer: Doc Nailin Li, Dept Medicine-Solna, Clinical Epidemiology Unit, Clinical Pharmacology Group
Earlier evaluation of the course Evaluation report
Course responsible Nailin Li
Institutionen för medicin, Solna
08-51773996
Nailin.Li@ki.se

Clinical Pharmacology Unit
Karolinska University Hospital-Solna
17176
Stockholm
Contact person Nailin Li
Institutionen för medicin, Solna
08-51773996
Nailin.Li@ki.se

Clinical Pharmacology Unit
Karolinska University Hospital-Solna
17176
Stockholm