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Title | Flow cytometry: from theory to application |
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Course number | 1496 |
Programme | Allergi, immunologi och inflammation (Aii) |
Language | English |
Credits | 1.5 |
Date | 2015-09-21 -- 2015-09-25 | Responsible KI department | Institutionen för medicin, Solna |
Specific entry requirements | |
Intended learning outcomes | Through this one-week course, the participants will acquire the knowledge of flow cytometry principles. They will know flow cytometric sample preparation and analysis, protocol set-up, as well as data interpretation. |
Contents of the course | The course covers basic knowledge and main applications of flow cytometry, and contains both lectures and laboratory/group work. Experts in different fields of flow cytometric application are invited to give the lectures. The participants will be able to learn: a) Principles of flow cytometry: working mechanisms of flow cytometer; molecular and cellular probes for flow cytometry; quality control; and safty procedures and intrument maintainance. b) Basic skills: flow cytometric protocol design; sample handling and storage; data processing and analysis, and data presentation. c) Major applications of flow cytometry in biomedical research: leukocyte phenotyping, endothelial cell analysis, stem cell analysis, apotosis, platelet measurement, cell function analysis (including membrane potential, oxidative metabolism, intracellular calcium mobilization, pH, and intracellular organelles), as well as nucleic acid analysis. |
Teaching and learning activities | The course includes 28 hr lectures and 12 hr hands-on lab work. |
Compulsory elements | The participants must attend hands-on lab sections during two afternoons. The students who have missed these sections can book extra lab section time within 4 weeks to compensate the absence. |
Examination | The exam will consist two parts: a 30-min written test on flow cytometric principles; and a 60 min hands-on lab work examining flow cytometric lab skills |
Literature and other teaching material | The course will provide organized handouts from all lectures. |
Number of students | 20 - 32 |
Selection of students | The course used to receive far more applications than the maximal course slots of 32. Selection of the participants is thus needed and made according to: 1) How relevant is flow cytometry to the thesis project? 2) How urgent is the flow cytometry training for her/his project work? 3) Has the student applied for the course before? |
More information | The course will be held during week 39 (Sept 21-25, 2015; 9:00-16:30). Lecture hall booked: Block Salen, T4:00 at Karolinska University Hospital-Solna. Lectures will be given by 14-15 lecturers, usaually half of them from institutions outside KI, with one or two from abroad. All the lecturers are well-established flow cytometrists on their lecture subjects. The course has been given 1-2 times/year at KI for 14 years. It has been highly appreciated by the participants through the years, with a median general satisfaction score never below 8 (out of 9; referred to paper-based course evaluation between 2001-2013), and highly remarked with the websurvey (2014). |
Additional course leader | Course organizer: Doc Nailin Li, Dept Medicine-Solna, Clinical Epidemiology Unit, Clinical Pharmacology Group https://websurvey.textalk.se/rep.php?ID=67661 |
Latest course evaluation | Course evaluation report |
Course responsible |
Nailin Li Institutionen för medicin, Solna 08-51773996 Nailin.Li@ki.se Clinical Pharmacology Unit Karolinska University Hospital-Solna 17176 Stockholm |
Contact person |
Nailin Li Institutionen för medicin, Solna 08-51773996 Nailin.Li@ki.se Clinical Pharmacology Unit Karolinska University Hospital-Solna 17176 Stockholm |