Kurskatalog forskarutbildning - VT24

    Startsida
  • Ansökan kan ske mellan 2023-10-16 och 2023-11-15
Application closed
Skriv ut
Titel Basic Training in Flow Cytometry
Kursnummer 2747
Program 1-Ingår i flera program
Språk Engelska
Antal högskolepoäng 1.5
Datum 2015-01-19 -- 2015-01-23
Kursansvarig institution Institutionen för medicin, Huddinge
Särskild behörighet None
Kursens lärandemål After completed course, the students will be able to:

1- Describe core concepts in flow cytometry
2- Critically examine and implement an experimental design of flow cytometry-based cell analysis according to Good Cytometry Practice (GCP)
3- Practically perform data analysis on a flow cytometer
4- Reflect upon and discuss pitfalls in flow-cytometry and data interpretation
Kursens innehåll This is a highly interactive ""hands-on"" course intended for both complete novices and experienced cytometrists with interest to refresh and enhance their knowledge of modern flow cytometry. The generic nature of this course ensures the translation of the aquired knowledge to any brand of flow cytometry instrument and to a large field of biological sciences.

Starting with the basics and progressively advancing towards more sophisticated applications. Students will be exposed to fundamental theory necessary for proper experiment design and data interpretation. To ensure meaninful learning, lectures will be followed up by workshops and ""hands-on"" practical sessions in front of flow cytomter instruments.

Topics to be covered during the course include:

-Planning my experiment according to Good Cytometry Practice (GCP): (Instrument, reagent selection, assay optimization/validation, quality controls)
-Running my experiment according to GCP (sample preparation and instrument setup and compensation)
-Analysing my experiment and generating data (Multidimensional data analysis: gating, data display, statistics and trouble-shooting)
-Preparing my data for publication
Arbetsformer The pedagogic learning activities of this course include lectures, problem-based group discussions/workshops. Group work will be conducted where the students actively discuss and integrate information and review articles from the lectures. The course also includes practical exercises and demonstrations on different flow cytometry instruments.

Obligatoriska moment Full time participation in the lectures, workshops, group discussions and practical sessions is mandatory. Absence is compensated according to the instructions of the course director.
Examination The examination will consist of an individual written exam where students will analyse and reflect upon selected cases from both good and bad flow cytometry praxis. The individual practical performance of each student will be evaluated separately.
Kurslitteratur och övriga läromedel The course literature will consist of both original and review articles. Handouts from the lecturers will also be included.
Antal studenter 5 - 25
Urval av studenter Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
Övrig information The course is co-organised between three doctoral programmes: Regenerative medicine, Infection biology, and Allergy, immunology and inflammation (Aii).

This is a highly interactive ""hands-on"" course intended for both complete novices and experienced cytometrists with interest to refresh and enhance their knowledge of modern flow cytometry. Invited national and international experts in the field of flow cytometry and its application will take part of the different teaching occasions. Lectures and practical sessions are both held at the WIRM flow cytometry facility at the Huddinge campus (Hälsovägen 7, Novum Level 4 hiss G, Follow sign to HERM/WIRM flow cytometry facility). (Note: A free shuttle service is available for KI students and staff to commute between Solna and Huddinge, see ki.se for more info).
Ytterligare kursledare Dr. Iyadh Douagi, Karolinska Institutet Center for Hematology and Regenerative Medicine (HERM). Department of Medicine Huddinge. Karolinska University Hospital Huddinge.
Senaste kursvärdering Kursvärderingsrapport
Kursansvarig Iyadh Douagi
Institutionen för medicin, Huddinge
08-585 83 602
iyadh.douagi@ki.se

HERM, Hälsovägen 7, Novum plan 4 hiss G

SE-14157
Huddinge
Kontaktpersoner -